Gene Information

Name : topA_2 (PFL_4672)
Accession : YP_261755.1
Strain : Pseudomonas protegens Pf-5
Genome accession: NC_004129
Putative virulence/resistance : Unknown
Product : DNA topoisomerase I
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0550
EC number : 5.99.1.2
Position : 5393086 - 5395002 bp
Length : 1917 bp
Strand : +
Note : identified by similarity to SP:P39814; match to protein family HMM PF01131; match to protein family HMM PF01751

DNA sequence :
ATGAAGCTCATGATCATCGAAGCGCCGGGCAAGCTGAAAAAGCTGGCCCCGATGCTCAAGAAACTACGGCCGGGCGAGCA
TTGGGAGGTGGTCGCCAGTGGCGGCCATATAAGGGATCTTCCCGAGAAGGGACACGACGACACGATGATTATTGCCGGGG
TGCTCAAGGATCTGCGCCCGGTGTACACGATCAGGGACAAGAGTTCCGGCAGGATCAAAACGATGCGCGAAGCTCTTGCC
CGGGCAACAGAAATCTACATCGCAACGGACCCCGATCGGGAAGGTGAAAGTATCGCGTGGCACATCCAGCAGACACTGCG
GATCCGCGACTACAAGCGCATTGCCTTTAACGAGGTCACCCACACGCGGGTGAAGGAGGCGCTGGCCAATCCGCGCAAGA
TTGACGCCCAGCTGGTGGCTTCACAGGAGTGCCGGCGAGTCCTCGATAGGCTCGTTGGCTATCTGGTGACTGGTGAGCTA
CGGAGGGTGCTTGGACTGCCCACAACAGCAGGCAGGGTACAGTCGGTTGCCGTTTACCTGGTGGTGTTGAGAGAGCGCGA
GATCCGCGACTTTAAGGTCGTTCACCACTTTGGCGTTCGCCTGCAGTTCCAGAACCAAACGACTGGTAGCTCCTGGACGG
CTGACTGGGTGACCGTTCCAGACTTCACCTCCAAGGAACACCCGTATGTGCAGGATGGCACCCTGGCTGCACAGGTCGCC
GGGGTCAGGAACGTCATTGTGGAGTCCTGCGAAGATACCAAGGAACGCCGAGCCCCCTACCCTCCCTACATATCCTCAAC
CCTTCAACAAGCGGCGAGCGTTGCGTTGAAGTGGGACCCTGACAAGACCATGCAGATCGCTCAGAGGCTGTATGAACAGG
GCGTGATCACCTACCACAGAACAGACAACCCGAACGTCGGCGACGATGTCATGGGTGATATCCGCGCTGTTGCACAAGCT
CTTGGTATACCAGCGGTTGAGAAGCGAAGGGAGTTTAGGGCAGCGGAGGGAGCCCAACTGGGCCACCCCGCGATCGCCCC
AACGACCTGGGCGGACGAGGTTGCAGGGGAAGATGATGAGCAGCGTCAGCTGTACAAACTCATCCGGATCCGGGCTCTGG
CAAGTCAGCTCGCTGATGCCATCTATGACGCCAGGATTGTGAAGCTCTTGGCGAAAGGCCCAGATCAACGCCCTCTGCGT
TTCAGTGGATCTTGTAGGACTGTTGCTTACCGCGGATGGATGCAGCTTCTGGCGGGAGACGACACGGATGAAGAAGGCGC
TGAAGCAGCCAACAACGAGATCCCAAAGCTGGAGCCAAAGCAGATCTTGCCGGTTCTGAATGGCCAAGTGCTGAACCAGA
AAACCAAAGCTCCGCCGCGCTACACGAAAGCCAGTCTGGTCAAGGAGCTCGAACGTCAGGGCATTGGGCGGCCGGGTACT
TTCGCCTCGATCATCAAGAACATCACCAGCAGATCCCAAGTGATTGAGGAGAAACGAAAGCTTATACCAGGTCCGCTCGG
CGAGCAGACCATTGCCCAACTCGAAGGCCGGTTTAGCTTCGTGCAGACTCAGTTCACCAGCAGTATGGAACGGGCCTTAG
ACAAGATTGCTGACGGCCAGAGTACCTACAACGCAGTGGTTTCCAAGTTTTACGAACACCTGCAGAGGGAACTGTCATCG
CTCAAAGACGCTCCTTCACTCCGTATGCAATCTGCACGCACGAGCGCTTCAACAACGACAGGGCCTGAACATCAGTACCT
GTGCGGCAAATGCGGCAAGCACCTGGTCAGACGACAGAAACGAGGTGAAGGCGGGTATGACTTCTGGGCGTGTTCCGGGT
ACAAGGATACGGGATGCAAGGTCAGCTACCCGTCGGTCAAAGAAAAGCCCGACCTGACTAAGGCCAGAGGCCTCTAG

Protein sequence :
MKLMIIEAPGKLKKLAPMLKKLRPGEHWEVVASGGHIRDLPEKGHDDTMIIAGVLKDLRPVYTIRDKSSGRIKTMREALA
RATEIYIATDPDREGESIAWHIQQTLRIRDYKRIAFNEVTHTRVKEALANPRKIDAQLVASQECRRVLDRLVGYLVTGEL
RRVLGLPTTAGRVQSVAVYLVVLREREIRDFKVVHHFGVRLQFQNQTTGSSWTADWVTVPDFTSKEHPYVQDGTLAAQVA
GVRNVIVESCEDTKERRAPYPPYISSTLQQAASVALKWDPDKTMQIAQRLYEQGVITYHRTDNPNVGDDVMGDIRAVAQA
LGIPAVEKRREFRAAEGAQLGHPAIAPTTWADEVAGEDDEQRQLYKLIRIRALASQLADAIYDARIVKLLAKGPDQRPLR
FSGSCRTVAYRGWMQLLAGDDTDEEGAEAANNEIPKLEPKQILPVLNGQVLNQKTKAPPRYTKASLVKELERQGIGRPGT
FASIIKNITSRSQVIEEKRKLIPGPLGEQTIAQLEGRFSFVQTQFTSSMERALDKIADGQSTYNAVVSKFYEHLQRELSS
LKDAPSLRMQSARTSASTTTGPEHQYLCGKCGKHLVRRQKRGEGGYDFWACSGYKDTGCKVSYPSVKEKPDLTKARGL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
topA AAP84217.1 topoisomerase I Not tested PAPI-1 Protein 2e-179 56
unnamed ABR13436.1 DNA topoisomerase I Not tested PAGI-5 Protein 0.0 56
top ACP28824.1 DNA topoisomerase I Not tested AbaR5 Protein 7e-111 46
topA ADI24145.1 DNA topoisomerase I Not tested AbaR1 Protein 5e-111 46