Gene Information

Name : pchD (PFL_3496)
Accession : YP_260599.1
Strain : Pseudomonas protegens Pf-5
Genome accession: NC_004129
Putative virulence/resistance : Virulence
Product : salicyl-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 4027168 - 4028835 bp
Length : 1668 bp
Strand : -
Note : Previously identified and characterized. See Youard et al 2007 (PMID: 17938167) for more information.; identified by similarity to GB:AAD55799.1; similarity to GB:ABW70812.1; match to protein family HMM PF00501

DNA sequence : 
ATGTCCACTTTCGATGACCTCAAGGATTGCCCCTCTTGGCCCGAAGACTTCGCCCAGCGCTACCGCCAGGCCGGCTACTG
GCGCGACGAAACCTTTGGCGACCTGCTGCGCAGCGCCGCCCAGGCCTTTGCCGAGCGTGAAGCACTGACCGAGGGCGAGC
AGCACCTGAGCTACCGGCAACTGGACCTGCGGGTCGACCAACTGGCCGCCGGCTTATACCGGCTGGGTCTGCGGGCCGGC
GACAACGTGGTGCTGCAACTGCCCAACAGTGCGGCCTTCGTCGAAGTCTGCTTTGCCCTTTACCGCCTCGGGGTGCGGCC
GATCTTCGCGCTGCCGGCCCACCGGCACCTGGAAATCGGCCGCTTCTGCGAGTTCGCCCAGGCCCGGGCCTACTTCTGCG
CCGACCGGGACGCCAGCTTCGACTATCGGGCCATGGCCCGCGACCTCAAGGACCGCAACCCGCAGCTGGAGTGGGTGGTA
GTCGCCGGCGAGGCCGAGGAATTCACGGCCTTGCACAGCCTGTATGAGCCGGCGCCTGTGCGTACCTTCCAGTCCCCCAG
CGCCGACGCGGTGGCCTGTTTCCAGCTCTCCGGCGGCTCCACCGGGGTGCCCAAGCTGATCCCACGCCGGCACCACGAAT
ACCTCTACAACCTGCGGGCCAGTGCCGAGCGCTGTGGCCTGTCCGAGGCCAGCGTGTACCTGGTGGCGCTGCCCATGGCG
CACAACTTTCCCATGTGCTGCCCAGGGTTCATCGGCACCTTTTCGGTGGGCGGGCGGGTGGTGCTGAGCCCCTCGCCGAG
CCCGGAAGTCTGCTTCGAACTGATCGAGCGCCAGGGGGTGACCCACACCGCGCTGGTGCCGCCCCTGGCCCTGGTCTGGC
TGGAAGCGGCCCAGGCCCGCGGGCGTGGCCTGGCGCCGTTGCAACTGCTGCAAGTGGGCGGCGCCAAACTCAGCTACGAG
GCCGCAAGGCGCATCGAACCGGTGCTCGGCTGCCGCCTGCAGCAGGTGTTCGGCATGGCCGAGGGGCTGATCTGCTACAC
CGACCCCGAGGACCCGCCACAGCGGGTGCTGCACACCCAGGGCCGGCCGCTGTCGCCGGCGGACGAAATTCGTGTGGTGG
ACGAGCACGACCAGCCGGTGCCGGTGGGGCAGGTGGGGCAGTTGCTGACCCGCGGCCCTTACACCATCCGCGGTTACTAC
CGTTACCCCGAGCACAACGCCCAGGCCTTCACGGCCGATGGTTTCTACCGCACCGGCGACCGGGTCATGCTCACTGCCGA
CGGCTACCTGATGGTGGAAGGGCGCGACAAGGACCTGATCAACCGCGGGGGCGAAAAGATCGCTGCCGAAGAAGTGGAAA
ACCTGCTGCTCAGCCACCCGTCGGTGGCCGACATCGCCCTGGTGGCGATGCCTGATGCCTTCCTGGGCGAACGCACCTGC
GCCTTCGTCATCCCCCGCGGCACCGCGCCCCGGGCCCCGGAGCTGCTGCGTCACCTAAGGGCCCAGGGCCTGGCGGCGTT
CAAGCTGCCGGACCGCTTCGAGTTCATCCCGGCCTTCCCCCAGACCGGGGTCGGCAAGGTCAGCCGCAAGCACCTGCGCG
AGGCGATCCAGGCCCTGTACTTCGGCGCCCAGGCCGAACCCCTGGAGGGAAGTGACGCCCGTGGCTGA

Protein sequence : 
MSTFDDLKDCPSWPEDFAQRYRQAGYWRDETFGDLLRSAAQAFAEREALTEGEQHLSYRQLDLRVDQLAAGLYRLGLRAG
DNVVLQLPNSAAFVEVCFALYRLGVRPIFALPAHRHLEIGRFCEFAQARAYFCADRDASFDYRAMARDLKDRNPQLEWVV
VAGEAEEFTALHSLYEPAPVRTFQSPSADAVACFQLSGGSTGVPKLIPRRHHEYLYNLRASAERCGLSEASVYLVALPMA
HNFPMCCPGFIGTFSVGGRVVLSPSPSPEVCFELIERQGVTHTALVPPLALVWLEAAQARGRGLAPLQLLQVGGAKLSYE
AARRIEPVLGCRLQQVFGMAEGLICYTDPEDPPQRVLHTQGRPLSPADEIRVVDEHDQPVPVGQVGQLLTRGPYTIRGYY
RYPEHNAQAFTADGFYRTGDRVMLTADGYLMVEGRDKDLINRGGEKIAAEEVENLLLSHPSVADIALVAMPDAFLGERTC
AFVIPRGTAPRAPELLRHLRAQGLAAFKLPDRFEFIPAFPQTGVGKVSRKHLREAIQALYFGAQAEPLEGSDARG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-109 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-109 50
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 5e-108 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 8e-108 50
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-108 50
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 7e-108 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-108 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-108 50
ybtE CAA21394.1 - Virulence HPI Protein 7e-108 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pchD YP_260599.1 salicyl-AMP ligase VFG0168 Protein 1e-154 61
pchD YP_260599.1 salicyl-AMP ligase VFG0932 Protein 6e-108 46
pchD YP_260599.1 salicyl-AMP ligase VFG1817 Protein 2e-98 46