Gene Information

Name : lysS (tll0212)
Accession : NP_681003.1
Strain : Thermosynechococcus elongatus BP-1
Genome accession: NC_004113
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 191385 - 192905 bp
Length : 1521 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGCTGACGTGGGGGCAACGGGAGCCGAAATTCGGGCCACACGGATTGAGAAAGCAAAAACATTGCAAGCACAGGGAAT
GAGCCCCTATGCCTATCGTTGGGAGCGCACCCATACTGCAGCATTGCTGCAAGACAAATATGCCCACTTGGCCGCTGGCG
AAGCGGTGGGCGATCGCGTGAGTGTGGCCGGTCGCATTATGGCGCGGCGCATCTTTGGCAAGCTGGCCTTTTTTACGCTT
CAGGATGACAGCGGCACGATTCAGCTTTACCTCGATAAGCAAACCATTAGCCAAACCATGGGGGAAGCCGCCTTTGCTGA
TTTGAAACACCTTACTGATGTCGGCGATATCCTTGGTGCGGTGGGCACCCTCAAGCGCACTGAGAAGGGCGAACTGTCGG
TTGTGGTGGAATCCTACACCATGCTGACAAAATCATTGTTGCCCCTACCGGACAAATGGCACGGCCTCACAGATGTGGAA
AAGCGCTATCGTCAGCGCTATGTGGATCTCATCGTCAACCCCCAAGTGCGTGACACATTCCGCAAGCGAGCACTGATCAC
CGCCGCCATTCGCCGCTACCTCAATGAGCAGGGCTTTATTGAAATTGAAACACCTGTTCTCCAGGTGGAAGCTGGGGGTG
CCGAGGCACGTCCTTTTATTACCTACCACAACACCTTGGAGATGCAGCTTTATTTGCGCATTGCCACCGAACTCCACCTG
AAGCGCCTCATTGTCGGTGGGTTTGAGAAGGTGTATGAACTGGGGCGCATCTTTCGCAATGAGGGCATCTCAACCAAGCA
CAACCCTGAATTTACGTCCATTGAGGTCTATCAGGCCTACGCCGACTATAACGATATGATGACGCTGACGGAGGCGATTA
TTACCACAGGGGCGATGGAGGTGTTGGGCACCCTCAAAATCACCTATCAAGGGGAAACAATTGATCTGACACCCCCATGG
CGACGGGTGACAATGCACGATGCGGTGTTGGCAGCTACTGGAATTGACTTTCGCCAATTGGGGGATCTGACCGCGGCTAA
AATAGCCGCTCAAAAGGTAGGAGTAAAAGACTTAGACACCTGTGACACGATTGGCCGAGTGCTCAATGAAGTGTTTGAGC
AAATCGTTGAACCTACATTGATTCAGCCCACCTTTGTTCTCGACTACCCTGTGGAGATTTCTCCCTTGGCCAAGCCCCAC
CGCAGTCAACCGGGACTGGTGGAGCGATTTGAATTATTTATCGTCGGCCGCGAGCATGCCAATAGTTTTTCGGAGTTAAC
GGATCCTTTGGATCAACGGCAGCGCTTGGAGGAACAAGCGCGGCGCAAAGCGGCTGGGGATTTGGCAGCCCATAGTGTTG
ATGAAGATTTTCTCACCGCCTTAGAACATGGAATGCCACCCACAGGAGGTCTAGGAATTGGCATTGACCGCTTGGTCATG
CTGCTAACGGACTCGCCAAGTATTCGTGATGTGATTGCTTTCCCGCTGTTACGTCCTGAATCGGCGGGTGGTCAGGTTTG
A

Protein sequence :
MADVGATGAEIRATRIEKAKTLQAQGMSPYAYRWERTHTAALLQDKYAHLAAGEAVGDRVSVAGRIMARRIFGKLAFFTL
QDDSGTIQLYLDKQTISQTMGEAAFADLKHLTDVGDILGAVGTLKRTEKGELSVVVESYTMLTKSLLPLPDKWHGLTDVE
KRYRQRYVDLIVNPQVRDTFRKRALITAAIRRYLNEQGFIEIETPVLQVEAGGAEARPFITYHNTLEMQLYLRIATELHL
KRLIVGGFEKVYELGRIFRNEGISTKHNPEFTSIEVYQAYADYNDMMTLTEAIITTGAMEVLGTLKITYQGETIDLTPPW
RRVTMHDAVLAATGIDFRQLGDLTAAKIAAQKVGVKDLDTCDTIGRVLNEVFEQIVEPTLIQPTFVLDYPVEISPLAKPH
RSQPGLVERFELFIVGREHANSFSELTDPLDQRQRLEEQARRKAAGDLAAHSVDEDFLTALEHGMPPTGGLGIGIDRLVM
LLTDSPSIRDVIAFPLLRPESAGGQV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 6e-110 49
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 2e-86 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS NP_681003.1 lysyl-tRNA synthetase VFG1668 Protein 4e-110 49