Gene Information

Name : pykA (BCE_4729)
Accession : NP_981022.1
Strain : Bacillus cereus ATCC 10987
Genome accession: NC_003909
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 4383302 - 4385059 bp
Length : 1758 bp
Strand : -
Note : identified by match to protein family HMM PF00224; match to protein family HMM PF00391; match to protein family HMM PF02887; match to protein family HMM TIGR01064

DNA sequence :
ATGCGTAAAACTAAAATTGTATGTACTATAGGTCCTGCTAGTGAAAGTATTGAAAAATTAGAGCAATTAATCGAAGCAGG
TATGAACGTTGCTCGTTTAAACTTCTCTCATGGTAGCCATGAAGAACACGGCGCTCGTATTAAAAACATTCGTGAAGCTT
CAAAGAAAACTGGTAAAACAGTTGGTATCTTACTTGATACAAAAGGTCCAGAAATCCGTACTCACGACTTCGTAGACGGA
CAAGCTGAGCTTGTAACAGGTGCAGAAGTTGTTATTTCTACTGAGCAAGTATTAGGTACTGCAGAGAAGTTCTCTGTATC
TTATGCTGGTCTTTATGACGATGTAAACCCAGGTTCTCGCATTCTAATCGATGATGGTCTTATCGAGCTAGAAGTAATCG
AAAAAGCTGACGGAAACATCCGTACAAAAGTTCTTAACAGCGGAACTGTAAAAAATAAAAAAGGTGTTAACGTACCAAAC
GTAAGCATTAAGCTTCCTGGTATCACTGAAAAAGACGTAAAAGATATCATCTTCGGTATCGAGCAAAAAGTTGATTTCAT
CGCAGCTTCATTCGTACGTAAAGCAGCTGACGTATTAGAAATCCGTGAATTACTAGAAGAGCATAACGCTCAATACATCC
AAATCGTACCAAAAATCGAAAACCAAGAGGGTATCGACAATATCGATTCAATCTTAGAAGTTTCTGACGGTTTAATGGTA
GCTCGTGGTGACATGGGTGTAGAAATTCCACCAGAAGAAGTACCATTAGTACAAAAACGTCTAATCAAAAAATGTAACGT
GTTAGGTAAACCAGTTATTACTGCAACACAAATGTTAGATTCTATGCAACGTAACCCACGTCCAACTCGTGCGGAAGCAA
GTGACGTAGCTAACGCAATCTTCGATGGTACAGACGCAATCATGCTTTCAGGTGAAACAGCTGCTGGACAATACCCAGTA
GAAGCAGTAACAATGATGGCTAACATTGCAGTACGTGTTGAAAAATCATTACAATATGAAGATATGTTCAAAAAACGTAT
TAAAGAGTTCACTCCAACAATTACAGATGCAATTAGCCAATCTGTTGCGCATACAGCACTTGCTCTTGATGTAGCTGCAA
TCGTAGCTCCAACAGAAAGTGGATATACTGCGAAAATGATCTCTAAATACCGTCCAAAATCTCCAATCGTAGCTGTAACA
TCTGACGAGCAAGTAGGACGTCGTCTTGCGCTTGTTTGGGGTGTACAAGCATTTATGGCTGAGAAGCGCGCAGCTTCTAC
TGACGAAATGTTAGATACAGCGATTCAAACAGGTATGGATGCAGGTCTAATCGGACTTGGAGACACTGTAGTAATCACTG
CTGGTGTTCCAGTTGCTGAAACTGGTACAACAAACTTAATGAAAATCCACGTTGTTGGTGAAGAAGTTGCTAAAGGACAA
GGAATCGGTCGTAAAGCTGCAAAAGGTAAAGTAGTTGTAGCGAAAACAGCTGCTGAAGCTGTAGCGAACGTAAACGAAGG
TGATATCCTTGTTACAACAAGCACTGATAAAGATATGATCCCTGCAATCGAAAAAGCTGCTGCTCTAGTTGTAGAAGAAG
GCGGTCTAACAAGCCATGCGGCTGTTGTAGGTGTATCAATCGGTATTCCTGTTATCGTTGGTGTAAACGGCGTAACAGCA
ACTTTAAAAAATGGCCAAGAAGTAACAGTTGATGCAGCACGCGGAATTGTTTATAATGGACATGCGGAAGTGCTATAA

Protein sequence :
MRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKTVGILLDTKGPEIRTHDFVDG
QAELVTGAEVVISTEQVLGTAEKFSVSYAGLYDDVNPGSRILIDDGLIELEVIEKADGNIRTKVLNSGTVKNKKGVNVPN
VSIKLPGITEKDVKDIIFGIEQKVDFIAASFVRKAADVLEIRELLEEHNAQYIQIVPKIENQEGIDNIDSILEVSDGLMV
ARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQYPV
EAVTMMANIAVRVEKSLQYEDMFKKRIKEFTPTITDAISQSVAHTALALDVAAIVAPTESGYTAKMISKYRPKSPIVAVT
SDEQVGRRLALVWGVQAFMAEKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNLMKIHVVGEEVAKGQ
GIGRKAAKGKVVVAKTAAEAVANVNEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHAAVVGVSIGIPVIVGVNGVTA
TLKNGQEVTVDAARGIVYNGHAEVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 4e-98 50
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-98 50
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-98 50
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 3e-98 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykA NP_981022.1 pyruvate kinase VFG0479 Protein 7e-99 50