Gene Information

Name : MM_0715 (MM_0715)
Accession : NP_632739.1
Strain : Methanosarcina mazei Go1
Genome accession: NC_003901
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 861276 - 862709 bp
Length : 1434 bp
Strand : +
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGGAAATCCCGGATCATAAAACAAAGATCGTCTGTACAATAGGACCTGCCTCTTCTTCTGAAGAGATGATAAGGAAGCT
TATGCTTGCGGGCATGAACGTGGCAAGGATAAATTTTTCTCACGGAGACTTTGAGAGCCACTCAAGGGTTGTCCGAATTA
TCCGTAAGGTTGCAGATGAACTTGGCAGGACAATTGCAATCCTTGCAGACCTGCCTGGCCCGAAAATACGCATAGGCAAA
CTCGAAAAAGAGCCTGTTATGCTCCATAAAGGGAACCCGATAACCCTTACAATTGACGACACTCCGGGAAACGAAGAGAG
AATCCCTGTCAGTTACAAACAGCTTCCGGAAAGCGTAACCCCTGGGAGCCTTATCTACCTCAGTGACGGGTTCATACAGC
TCCTCTGCAAGGAGGTTACAGGAAAAGACGTTCTCTGTGAAGTCCTTATCGGAGGAGAGCTTTATTCGCACAAAGGGCTG
AACCTTCCAGGGGCAAAGATCTTTCTTGACGCTGTGACTGAAAAAGATTTCAGGATCCTGGAATTTGCCCTTGAAGAGGA
CATTGACACTTTCAGCATCTCTTTTGTAGAAAATGCAGAAGATATCCGAAAAGTAAGAAATTTTGCCGCTTCCAGAGGAA
AGCAGGTAAATATAGTATCAAAAATAGAACGCAGGCAGGCTGTAGAAAATATAGGAGAAATCCTTGACGAGACCGATGCC
CTTATGGTTGCAAGGGGAGACCTGGGAGTTGAAATTCCTATCCAGGAAGTCCCTTCGGTCCAGAAAGAACTTATCCAGAG
GGCAAAGCTCCTTGGGGTTCCTGTGATTACTGCAACCCATATGCTGGCTTCCATGACAGATAATATAAGACCCACGCGTG
CCGAAGCCACGGATGTTGCAAACGCTATCCTTGACGGCACGGATGCTGTTATGCTCTCGGAAGAAACAGCAGTCGGAAAC
TACCCCGTGGAAGCTGTCGAGATGATGGCAAAGATTGCAAAGACCACTGAAAACTGGCGCTCCCGGACTAAATGGGGGCT
TGACACAATGATCAAATCCATAACTGCCAGGGAGATGTCAGTGGATGAGGTAATCACCTTACAGGTTTATGAAGCCCTGC
AGAAACTGCCTGTTGCAGCCGTACTTACCCCGACAAGGAGCGGAGCGACTCCCCGCAGGATTTCGCGCTTTAATCCTGAC
CCCTGGATTCTGGCTTTCAGCCGCGTTCCTAAGACCTGCAGGTTCCTTTCCCTGTCTTACGGGGTTTATCCTGTTGCCGT
AAACGAATCTATTGAAAGCTGGGAAAAAGAGACAACGGAAAGAACAAAAGAATTGGGATTTGCGAAAAGTAAAGATCTCG
TGATTTTTACCCAGGGCCCTGCTTCCGGAAAACCCGGAGGTACGAATATGCTCAAGATCCTGACCCTTGACTGA

Protein sequence :
MEIPDHKTKIVCTIGPASSSEEMIRKLMLAGMNVARINFSHGDFESHSRVVRIIRKVADELGRTIAILADLPGPKIRIGK
LEKEPVMLHKGNPITLTIDDTPGNEERIPVSYKQLPESVTPGSLIYLSDGFIQLLCKEVTGKDVLCEVLIGGELYSHKGL
NLPGAKIFLDAVTEKDFRILEFALEEDIDTFSISFVENAEDIRKVRNFAASRGKQVNIVSKIERRQAVENIGEILDETDA
LMVARGDLGVEIPIQEVPSVQKELIQRAKLLGVPVITATHMLASMTDNIRPTRAEATDVANAILDGTDAVMLSEETAVGN
YPVEAVEMMAKIAKTTENWRSRTKWGLDTMIKSITAREMSVDEVITLQVYEALQKLPVAAVLTPTRSGATPRRISRFNPD
PWILAFSRVPKTCRFLSLSYGVYPVAVNESIESWEKETTERTKELGFAKSKDLVIFTQGPASGKPGGTNMLKILTLD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 5e-69 41
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 4e-69 41
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 4e-69 41
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 1e-68 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
MM_0715 NP_632739.1 pyruvate kinase VFG0479 Protein 2e-69 41