Gene Information

Name : pyk (MA3890)
Accession : NP_618761.1
Strain : Methanosarcina acetivorans C2A
Genome accession: NC_003552
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 4783546 - 4785015 bp
Length : 1470 bp
Strand : +
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
GTGAATATAAACGTGAATATAAACGGCCGTGAAAATATGCAAATCCCTGACCATAAAACAAAGATCGTCTGCACCATAGG
CCCTGCTTCCTTCTCCGAGGAAGTCCTCAGGAAACTGGTGCTTGCGGGAATGAACGTTGCAAGGATTAACTTTTCCCACG
GAGACTTCGAGAGCCACGGAAAAGTTATCCGGAGGGTCCGAAAGGTTGCAGAAGAGCTTGACAGGACAGTTGCTATCCTT
GCCGACCTCCCAGGCCCGAAAATTCGCGTAGGCAAGCTCAAAAAAGAGCCGCTTATGCTCCATAAAGGGAACCGAATAAC
CCTTACCACTGACGAGACTTCTGGAAGCGAGGACCGCATCCCGGTCAACTACAAACAGCTCCCTGAGAGCGTCTCCCCGG
GAAGCCTTATCTACCTGAGTGACGGATTTATCCAGCTCCTCTGCCTGGAAATCTCGGGAAAAGATGTAGTCTGCGAAGTT
ATGGTCGGAGGACAACTCTACTCCCATAAAGGGCTGAACCTGCCAGGGGCAAAAATTTACCTGGACTCTGTGACGGAACA
TGACTTCAAAATCCTTGAGTTTGCCCTGAACGAAGAAGTTGACGCAGTCAGCATCTCTTTTGTGGAAAAAGCCGAAGATA
TCCGAAAAGTCCGGAATTTTGCTTCAACCATGGGAAAACCTGTATACGTTGTCTCAAAAATCGAACGGAGCCAGGCGGTC
CAGAATATAGAAGAGATCCTTGAGGAAACCGATGCCCTGATGGTTGCCAGAGGAGACCTGGGAGTTGAGATTCCTATCCA
AGAAGTACCTTCGGTCCAGAAAGAACTCATCCGGAGTGCAAAGCTCCTGAGCATCCCCGTAATTACTGCAACCCACATGC
TGGCCTCCATGACCGACAACATCAGGCCTACAAGGGCGGAGGCTACAGACGTTGCCAACGCCATCCTTGACGGGACGGAC
GCGGTCATGCTCTCGGAAGAAACCGCAGTTGGGAACTATCCCGTGGAAACTGTGGAGATGATGGCAAAAATTGCAAAAAC
AACAGAAAACTGGCGCTCCCGAACGAAGTGGGGACTTGACACAATGCTCAAGGGAATTACGGCACAGAAAATGTCAGTGG
ACGAGGTAATCGCTCTCCAGGTACACGAAGCCCTGCAAAAGCTTCCTGTAGCCGTTGTACTCACCCCAACCCGAAGCGGA
GCAACCCCGCGCAGGCTTTCCCGCTTCAAGCCTGAACCCTGGATCCTGGCTTTCACCCGCTTCCCGAAAACCTGTAGCTC
CCTTGCCTTGTCCTATGGGGTTTATCCGATAATAGTAAAAGAAGTCTCTGAAAACTGGGAAACGGAGACCACGGAAAAGG
CAAAGGAACTGGGATTTGCAAAAAGCGGAGAGCTCGTAGTTTTCACCCAGGGACCTGCTTCCGGAAAACCAGGAGGCACA
AACATGCTCAAGATCCTGACTCTGGACTGA

Protein sequence :
MNINVNINGRENMQIPDHKTKIVCTIGPASFSEEVLRKLVLAGMNVARINFSHGDFESHGKVIRRVRKVAEELDRTVAIL
ADLPGPKIRVGKLKKEPLMLHKGNRITLTTDETSGSEDRIPVNYKQLPESVSPGSLIYLSDGFIQLLCLEISGKDVVCEV
MVGGQLYSHKGLNLPGAKIYLDSVTEHDFKILEFALNEEVDAVSISFVEKAEDIRKVRNFASTMGKPVYVVSKIERSQAV
QNIEEILEETDALMVARGDLGVEIPIQEVPSVQKELIRSAKLLSIPVITATHMLASMTDNIRPTRAEATDVANAILDGTD
AVMLSEETAVGNYPVETVEMMAKIAKTTENWRSRTKWGLDTMLKGITAQKMSVDEVIALQVHEALQKLPVAVVLTPTRSG
ATPRRLSRFKPEPWILAFTRFPKTCSSLALSYGVYPIIVKEVSENWETETTEKAKELGFAKSGELVVFTQGPASGKPGGT
NMLKILTLD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-67 41
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 9e-68 41
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 9e-68 41
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 3e-67 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk NP_618761.1 pyruvate kinase VFG0479 Protein 4e-68 41