Gene Information

Name : pykA (CPE2149)
Accession : NP_563065.1
Strain : Clostridium perfringens 13
Genome accession: NC_003366
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : -
Position : 2460912 - 2462336 bp
Length : 1425 bp
Strand : +
Note : similar to gpu:AP001517_285 pyruvate kinase (EC 2.7.1.40) from Bacillus halodurans (584 aa); 50% identity in 474 aa overlap

DNA sequence : 
ATGCAAAAAACAAAAATGATATTTACAATTGGTCCTTCAAGTGATTCAGAAGAAATTTTAAGAGAGTTCATAAGAATTGG
TATGAATGCAGCTAGATTAAACTTCTCACATGGAGACCATGCAAGTCATAAAGAAAAAATAGAACTTATTAAGAGACTTA
GAAAAGAAGAAAAATCAGCTACAGCTATCTTATTAGACATTAAGGGTCCTAAAATAAGAACATATAATTTTAAAAATGGC
GAAGCTGAATTAAAAAATGGTGATGAATTCACATTCTCTTGCGGAGATGAAATATTAGGTGATAACACTAAATGTTCAAT
CTCTTATAAAGAACTATATGAAGACATAAAACCTGGTGGATCAATACTTGTTGATGATGGACTTTTAGAATTTAAAGTAA
AGGAAGTCCGTGGAACTGATATAATATGTGAAGTTATTGAAGGAGGTACTATAAAAGACCATAAAGGTGTAAATGTACCT
AATGTACCAATCAAACTCCCTGCAGTAACTGAAAAGGATAGATCTGACTTAATATTTGGTTGCGAGATGGAAGTAGACTT
TGTAGCAGCTTCTTTCATAAGAAAACCAGAGGATGTACTTGAAGTTAGAGAAATTCTAGACTCTCATGGTGGAAAAGATA
TAAAAATAATATCAAAAATAGAAAGCCAAGAAGGTGTAGATAACATAAAAGAAATAATTAAAGTTACTGATGGTGTAATG
GTTGCTAGAGGTGATATGGGAGTAGAAATTCCTATAGAAAATGTACCTATAATTCAAAAAAATATAATTAAGAAATGTAA
TCAAGCAGGGAAAATAGTTATAACGGCAACTCAAATGTTAGATTCTATGATAAGAAATCCAAGACCTACTAGAGCTGAAG
CTTCTGACGTATGTAATGCCATATTTGATGGTACAGATGCCATAATGCTTAGTGGAGAAAGTGCTTCTGGTAGCTTCCCT
ATAGAGGCAGCAATGACAATGTCTAGAATAGCAAAAAAAGCAGAAGCTAACTTAGACTATAATTATCTTTTAAGAAGATT
AAAAGATCCAAATCCAAATCCCGATGCCTTTGCAGATGCCATAAGTTATTCAGCATCAAAAACAGCTAGCAAATTCCCTA
CAAAAGCTATAGTAGCAGCCACTCAGACTGGTTCAACTGCTAAGATATTATCAAAATATAAACCTAGCTGTCCAATAATA
GCTATTACTCCATATGAAAAAGTTAGAAGAAGCTTAGCTTTAAACTTTGGAATAATTTCTAAGAAATGTGCTTATTTTAA
TAGTACAGATGAAATTATAGAGGAAGCTAGAAAAGTAGCTAAAGAATTTGAAATAGCTGAAACTGGAGATAATATAATGG
TAGCTGCTGGATTCCCAACATCAATAACTGGTGGAACAAACATGCTAAAAATTGAAAAAATATAA

Protein sequence : 
MQKTKMIFTIGPSSDSEEILREFIRIGMNAARLNFSHGDHASHKEKIELIKRLRKEEKSATAILLDIKGPKIRTYNFKNG
EAELKNGDEFTFSCGDEILGDNTKCSISYKELYEDIKPGGSILVDDGLLEFKVKEVRGTDIICEVIEGGTIKDHKGVNVP
NVPIKLPAVTEKDRSDLIFGCEMEVDFVAASFIRKPEDVLEVREILDSHGGKDIKIISKIESQEGVDNIKEIIKVTDGVM
VARGDMGVEIPIENVPIIQKNIIKKCNQAGKIVITATQMLDSMIRNPRPTRAEASDVCNAIFDGTDAIMLSGESASGSFP
IEAAMTMSRIAKKAEANLDYNYLLRRLKDPNPNPDAFADAISYSASKTASKFPTKAIVAATQTGSTAKILSKYKPSCPII
AITPYEKVRRSLALNFGIISKKCAYFNSTDEIIEEARKVAKEFEIAETGDNIMVAAGFPTSITGGTNMLKIEKI

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-92 47
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-92 47
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 1e-91 47
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-92 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykA NP_563065.1 pyruvate kinase VFG0479 Protein 7e-93 47