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Name : lysS (RSc1028) Accession : NP_519149.1 Strain : Ralstonia solanacearum GMI1000 Genome accession: NC_003295 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 1079620 - 1081152 bp Length : 1533 bp Strand : - Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGACCGAACCGAACACCCCGGCCGCACCCACTGGACAGGCGCAAGACGACAACCAGATCATGGCCGAGCGCCGCGAGAA GCTCGCCGCGCTGCGCCAGCAGGGCGTCGCCTATCCCAACGATTTCTGCCCGACCCACCACGCGGCCGACCTGCACACCC GCTACAGCGAGACCGACCAGCCCGCGCTGGAAGCGGCCAATGTCGAGGTCGCCCTCGCCGGGCGCATGATGCTCAAGCGC GTGATGGGCAAGGCCAGCTTCGCCACCGTGCAGGACGGCAGCGGCCAGATCCAGTTCTACATCACGCGCGACCGCGTGGG CGAAGACGTCTACGCCGCCTTCAAGCATTGGGACCTCGGCGACATCGTCGCCGCGCGCGGCGTGCTGTTCCGCACCAACA AGGGCGAGCTGTCGGTGCAGGTGCAGGAGCTGCGCCTGCTGTCGAAGTCGCTGCGTCCGCTGCCGGACAAGTTCCACGGC CTGGCCGACCAGGAAATGAAGTACCGCCAGCGCTACGTTGACCTGATCGTCTCGCCCGAGACGCGCAACACCTTCCGCGC CCGCACCAAGGCCATCGCCTCGCTGCGCCACCACATGAGCGATGCCGGCTTCATGGAAGTGGAAACGCCGATGCTGCACC CGATTCCGGGCGGCGCGGCGGCCAAGCCCTTCATCACGCACCACAACGCGCTGGACATGCAGATGTTCCTGCGCATCGCC CCGGAGCTGTACCTCAAGCGCCTGATCGTCGGCGGCTTCGAGCGCGTGTACGAGATCAACCGCAACTTCCGCAACGAGGG CGTGTCGCCACGGCACAACCCCGAGTTCACGATGATGGAGTTCTATGCAGCCTACACGGACTACCGCTGGCTGATGGACT ACACCGAGAACCTGATCCGCCAGGCCGCCATCGATGCCACCGGCAGCGCCGCCCTCAACTACCAGGGTCGCGAGCTGGAC CTGTCCAAGCCCTTCCACCGCCTGACCATCTGCCAGGCCATCCAGAAGTACGCGCCGCAGTACACCGACGCGCAACTGGC CGACGCCAACTTCCTGCGTGCCGAGCTCAAGAAGTTCAAGATCGACACCAACGCGCCGCAGTTCCTGAACGCCGGCGTGG GCACGCTGCAGCTCGTCCTGTTCGAAGAGACGGCCGAGTCGCAACTGTGGGAGCCGACCTTCATCGTCGACTACCCGGTC GAGGTGTCGCCGCTGGCACGCGGCTCGGACACGCTGCCCGGCATCACCGAGCGCTTCGAGCTGTTCATCACCGGCCGCGA GATCGCCAACGGCTTCTCCGAGCTGAACGACCCCGAAGACCAGGCCGAACGCTTCCGCAAGCAGGTCGAACAGAAGGACG CCGGCGACGAGGAAGCCATGTTCTACGATGCCGACTACATCCGCGCGCTGGAATACGGCATGCCCCCGACCGGCGGCTGC GGCATCGGCATCGACCGCCTGGTGATGCTGCTGACCGACAGCCCCAACATCCGCGACGTGATCCTCTTCCCGCACCTGCG CCGCGAAGACTGA Protein sequence : MTEPNTPAAPTGQAQDDNQIMAERREKLAALRQQGVAYPNDFCPTHHAADLHTRYSETDQPALEAANVEVALAGRMMLKR VMGKASFATVQDGSGQIQFYITRDRVGEDVYAAFKHWDLGDIVAARGVLFRTNKGELSVQVQELRLLSKSLRPLPDKFHG LADQEMKYRQRYVDLIVSPETRNTFRARTKAIASLRHHMSDAGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMFLRIA PELYLKRLIVGGFERVYEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDYTENLIRQAAIDATGSAALNYQGRELD LSKPFHRLTICQAIQKYAPQYTDAQLADANFLRAELKKFKIDTNAPQFLNAGVGTLQLVLFEETAESQLWEPTFIVDYPV EVSPLARGSDTLPGITERFELFITGREIANGFSELNDPEDQAERFRKQVEQKDAGDEEAMFYDADYIRALEYGMPPTGGC GIGIDRLVMLLTDSPNIRDVILFPHLRRED |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 4e-122 | 53 |
| lysS | YP_005163386.1 | lysyl-tRNA synthetase | Not tested | Not named | Protein | 2e-76 | 41 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | NP_519149.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 2e-122 | 53 |
