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Name : lysS (BF3602) Accession : YP_213196.1 Strain : Bacteroides fragilis NCTC 9343 Genome accession: NC_003228 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 4250619 - 4252346 bp Length : 1728 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGAACATATTAGAACTAAGTGAACAGGAAATCATCCGACGTAACAGTCTGAATGAACTTCGCGCGATGGGCATCGAACC CTATCCCGCAGCAGAGTATGTAACCAATGCTTTCTCAACTGATATTAAAGCCGAATTCAAAGATGACGAGACACCCCGCC AGGTATCCGTAGCCGGTCGCATGATGAGCCGCCGCATAATGGGTAAAGCCTCTTTCATTGAATTGCAGGACTCTAAAGGC CGCATCCAGGTATATATCACCCGTGATGACATCTGCCCGGGAGAAGACAAGGAAATGTACAACACTGTGTTCAAACGCCT GCTCGACTTAGGCGACTTCATCGGAATCGAAGGCTTCGTATTCCGCACACAGATGGGCGAAATCAGTATCCATGCACAAA AGCTGACGGTGCTCGCCAAATCGATCAAACCACTGCCTATTGTTAAATACAAAGACGGCGTAACTTACGACTCTTTTGAA GATCCTGAATTGCGTTACCGCCAGCGCTATGTCGACCTGGCTGTCAACGAAGGTGTTAAAGATATTTTCATCAAACGCAG CAAAGTATACAGTTCCATGCGCGAATACTTCAACTCAAAGGGATACATGGAAGTGGAAACTCCGATCCTGCAGGCCATTG CCGGAGGAGCAGCCGCGCGTCCGTTCATGACTCACCACAATGCTTTGGATATTCCTTTATACATGCGAATCGCCAGTGAG CTTTACCTGAAACGCCTTATTGTCGGCGGTTTTGAAGGTGTATACGAGATCGGTAAAAACTTCCGTAACGAAGGTATGGA CCGTACACACAATCCAGAATTCACCTGTATGGAGATATATGTAGCCTACAAAGACTACAATTGGATGATGGAATTTACTG AAAAAATGATCGAAAAGATCTGTCTGGACGTAAACGGTACTACCGAAGTAAAAGTAGGCGACAACATCATCAATTTCAAA GCGCCCTATAAGCGTGTCACTATGCTGGGAGCCATCAAAGAGCACACCGGTTACGATCTGACAGGAATGAATGAAGAGCA GATTCGCGAAGTTTGCAAGAAACTGAACATGGAAATCGATGATACGATGGGTAAAGGTAAACTGATCGATGAGATCTTCG GTGAGTTCTGCGAAGGCACTTATATACAGCCTACATTCATCACGGATTACCCGATCGAGATGTCTCCTCTGACCAAGAAG CATCGTGACAACCCTGAACTGACAGAACGTTTCGAGTTGATGGTAAACGGTAAAGAGTTGTGTAACGCATATTCTGAGTT GAACGATCCGATCGACCAGTTGGAACGTTTCGAAGACCAGATGAAGCTGAGCGAAAAGGGAGATGATGAAGCGATGATCA TCGATAAGGACTTCGTCCGTGCATTGGAATATGGTATGCCTCCTACTTCGGGTATGGGTATTGGTATGGACCGTCTGACT ATGCTGATGACCGGTCAGTCGACCATTCAGGAAGTATTGTTCTTCCCGCAGATGCGTCCGGAGAAAGTCGTACCGAAAGA CAGTGCTTCCAAATTTATGGAACTGGGCATTGCCGAAGAGTGGGTACCTGTCATCCAGAAAGCAGGATACAATCAGGTGG CCGACATGAAAGAGGTTAATCCGCAGAAATTCCACCAGGATATTTGTGGCATCAACAAGAAATACAAATTGGAACTGACC AATCCGTCAGTTAACGATGTAGCCGAGTGGATACAGAAAATTAAATAA Protein sequence : MNILELSEQEIIRRNSLNELRAMGIEPYPAAEYVTNAFSTDIKAEFKDDETPRQVSVAGRMMSRRIMGKASFIELQDSKG RIQVYITRDDICPGEDKEMYNTVFKRLLDLGDFIGIEGFVFRTQMGEISIHAQKLTVLAKSIKPLPIVKYKDGVTYDSFE DPELRYRQRYVDLAVNEGVKDIFIKRSKVYSSMREYFNSKGYMEVETPILQAIAGGAAARPFMTHHNALDIPLYMRIASE LYLKRLIVGGFEGVYEIGKNFRNEGMDRTHNPEFTCMEIYVAYKDYNWMMEFTEKMIEKICLDVNGTTEVKVGDNIINFK APYKRVTMLGAIKEHTGYDLTGMNEEQIREVCKKLNMEIDDTMGKGKLIDEIFGEFCEGTYIQPTFITDYPIEMSPLTKK HRDNPELTERFELMVNGKELCNAYSELNDPIDQLERFEDQMKLSEKGDDEAMIIDKDFVRALEYGMPPTSGMGIGMDRLT MLMTGQSTIQEVLFFPQMRPEKVVPKDSASKFMELGIAEEWVPVIQKAGYNQVADMKEVNPQKFHQDICGINKKYKLELT NPSVNDVAEWIQKIK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 1e-100 | 45 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_213196.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 8e-101 | 45 |
