Gene Information

Name : pykA (lmo1570)
Accession : NP_465095.1
Strain : Listeria monocytogenes EGD-e
Genome accession: NC_003210
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 1608166 - 1609923 bp
Length : 1758 bp
Strand : -
Note : highly similar to pyruvate kinases; catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGAAAAAAACGAAAATTGTTTGTACAATTGGTCCTGCAAGTGAGTCAGTTGACACATTAGTTCAGTTGATTGAAGCAGG
AATGAACGTAGCACGCCTTAATTTCTCTCACGGAGATTTTGAAGAGCACGGTGCGCGTATTGTAAATATCCGTGAAGCAT
CGAAAAAAACTGGTAAACAAGTGGCTATCTTACTTGATACAAAAGGTCCAGAAATCCGTACAAACGACATGGTCGGTGGA
AAATTAGAATTCCAAACAGGTGATGTTGTACGTGTTTCTATGACTCCTGTTGAAGGAACAAAAGAAAAATTCTCTGTAAC
TTACGGAGAACTTTATGATGACGTAGAAATTGGTTCTTCCATTTTACTTGATGACGGCTTAATCGGTCTTGAAGTAATCG
AAAAAGACGAAGCTAATCGCGAACTAGTAACTAAAGTACTTAACCCTGGCGTACTTAAAAATAAAAAAGGTGTTAACGTA
CCTAACGTTTCTATCAACCTACCAGGAATCACAGAAAAAGATGCTGCTGATATCCGCTTTGGTTTAGAACAAGGTATCGA
CTTTATCGCTGCATCTTTCGTACGTCGTGCTACAGATGTTCTTGAAATTACTAAAATTTTAGAAGAGCATAACGCAACTC
ACGTACAAATCATTCCTAAAATCGAAAACCAAGAAGGCGTTGACAATATCGACGAAATCTTGCAAGTATCTCAAGGCCTA
ATGGTTGCTCGTGGTGACCTTGGTGTTGAAATTCCAGCTGAAGAAGTTCCGATTGTACAAAAAGAACTTATCAGAAAATG
TAATAAACTTGGTAAGCCTGTTATTACTGCAACACAAATGCTTGATTCCATGCAACGTAACCCACGTCCAACTCGCGCTG
AAGCAAGTGACGTAGCGAATGCGATTTTTGATGGAACAGATGCTATTATGTTATCCGGTGAAACAGCTGCTGGGGATTAT
CCAGTAGAAGCAGTTAAAATGATGGCGAAAATCGCTGTTCGTACAGAAGAAGTACTAGTTGCTCAAGATAAATTTGCACT
TAAACTTCACGAAAATACGGATATGACAGAAGCTATCGGTCAAGCAGTTGGCCATACTGCGAAAAACTTAAACGTTCAAA
CTATCGTTGCTGCGACTCAAAGTGGTCACACAGCGCGTATGATCTCTAAATATCGTCCAAAATCTCATATCGTTGCTGTA
ACATTCAACGAGCATGTATACCGTGGTTTAGCACTTTCATGGGGTGTTTATCCTCGTCTAGCTACTCCAGTTAGCAACAC
AGATGAAATGTTCGACCTTGCAGTGAAAGAATCTCTTGCTTCTGGCGTTGCAAAACAAGGCGACTTAATTATCATCACTG
CTGGTGTTCCAGTTACTGAAAGTGGAACAACAAACGTAATGAAAATCCAATTAATTGGTGAAAAAGTAGTTAAAGGCCAA
GGAATTGGCAGCAAATCTGTTATCGGTAAAGCAATCGTTGCTAAATCCAATGCAGAAGCACTTGAAAAAGCAGAAGAAGG
CGGAATCTTAATCGTTAAAACAACAGATAAAGAAATCCTTCCTGCATTCGAGAAAAGTGCTGCAGTTGTTGTGGAAGAAG
GCGGCTTAACTAGCCACGCAGCAGTTGTTGGAATCAACCTTGGCATTCCTGTTATCGTTGGTGCTAAAGATGCAACATCT
CTTGTAAAAGATGGTGAAATCATCACAGTTGATTCTCGTCAAGGTGTTGTATATAACGGCAAAACAGCAACACATTAA

Protein sequence :
MKKTKIVCTIGPASESVDTLVQLIEAGMNVARLNFSHGDFEEHGARIVNIREASKKTGKQVAILLDTKGPEIRTNDMVGG
KLEFQTGDVVRVSMTPVEGTKEKFSVTYGELYDDVEIGSSILLDDGLIGLEVIEKDEANRELVTKVLNPGVLKNKKGVNV
PNVSINLPGITEKDAADIRFGLEQGIDFIAASFVRRATDVLEITKILEEHNATHVQIIPKIENQEGVDNIDEILQVSQGL
MVARGDLGVEIPAEEVPIVQKELIRKCNKLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGDY
PVEAVKMMAKIAVRTEEVLVAQDKFALKLHENTDMTEAIGQAVGHTAKNLNVQTIVAATQSGHTARMISKYRPKSHIVAV
TFNEHVYRGLALSWGVYPRLATPVSNTDEMFDLAVKESLASGVAKQGDLIIITAGVPVTESGTTNVMKIQLIGEKVVKGQ
GIGSKSVIGKAIVAKSNAEALEKAEEGGILIVKTTDKEILPAFEKSAAVVVEEGGLTSHAAVVGINLGIPVIVGAKDATS
LVKDGEIITVDSRQGVVYNGKTATH

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 4e-106 51
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-106 51
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-106 51
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 4e-106 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykA NP_465095.1 pyruvate kinase VFG0479 Protein 1e-106 51