Gene Information

Name : lysS (LMOf2365_0240)
Accession : YP_012850.1
Strain : Listeria monocytogenes F2365
Genome accession: NC_002973
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 240787 - 242283 bp
Length : 1497 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGAGTAACGAGAATCATGAAGAACTAAATGACCAACTCATCGTCCGTCGCGAAAAAGTGGACACATTACGCGAAGAAGG
CATAGATCCTTTTGGCGAAAAATTCATCCGTTCCATCAGCCCGGAAGAAATTGAAACAAAATTCGCTGATAAATCAAAAG
AAGAACTTGAAGAAGCTGCTATTGAAGTTTCTGTAGCAGGTCGTATTATGACGAAACGTGTGAAAGGTAAAGTAGGTTTC
ACACATATTCAAGACCGTTTCCATCAATTACAAATCTATATCCGTAAAGATGCAATTGGCGAAGATGCTTATGCAGTCTT
CAAATTAGCGGATCTAGGTGACATTATTGGTATAAAAGGGACTATTTTCCGTACTAATACAGGTGAACTTTCTGTTAAAG
CAACGGAATTCACTTTGCTTTCCAAATCATTACGTCCACTTCCTGATAAATATCATGGCTTAAAAGACGTAGAACAACGC
TATCGTCAACGCTATTTAGACTTAATTACAAACGAAGAAAGCCAAAATCGTTTTGTGATGCGCAGTAAAATCTTGAAATA
CACTCGTGATTACATGGATAATCAAGGATTCCTAGAAGTAGAAACTCCAGTGCTTCATACAATTGCTGGTGGTGCTGCTG
CAAAACCATTTATTACGCACCATAATGCACTTGATATGGAATTATATTTACGAATTGCTTTAGAACTACACTTAAAACGC
CTAATCGTTGGTGGGATGGATAAAGTATACGAAATCGGCCGCGTTTTCCGTAATGAAGGAACCTCCACGCGTCATAACCC
AGAATTTACAATGTTAGAATCCTATGCAGCATACGAAGATTACGAAGATGTTATGGATTTGGTAGAAGGTTTAGTATCTA
CAGTATGCAAACAAGTAAATGGAACAACAGAAATAACATACGGTGAATACAATGTAGATCTAACTCCAAACTGGCGCCGT
ATCCACATGGCAGATGCTGTAAAAGAATATGTCGGAGTGGATTTCTGGAACGTAACTTCCGATGAAGAAGCACGCGAACT
AGCTAAAAAACATAATGTGCCAGTAACGGAGCACATGACATACGGGCACATTCTTAATGAGTTTTTTGAAACATACGTAG
AAGAAAAGCTTATTCAACCAACCTTTGTTTACGGACATCCGGTGGAAATTTCACCATTAGCGAAGAAAAACAAAGAAGAT
GACCGTTTTACTGACCGTTTTGAATTATTTATCGTTGGTCGTGAACATGCAAATGCATTCTCGGAATTAAACGATCCAAT
TGATCAAAGAGAGCGTTTTGAAGCGCAAATGAAAGAACGTGAGCAAGGTAATGATGAAGCGCACGGAATGGATGCAGATT
TCCTTGAAGCTTTAGAATACGGTCTACCGCCGACTGGTGGACTTGGAATAGGTATCGATCGTCTAGTAATGCTATTAACA
GATGCCCCGTCTATTCGTGATATCCTATTATTCCCAACTATGAAACACCGCGACTAA

Protein sequence :
MSNENHEELNDQLIVRREKVDTLREEGIDPFGEKFIRSISPEEIETKFADKSKEELEEAAIEVSVAGRIMTKRVKGKVGF
THIQDRFHQLQIYIRKDAIGEDAYAVFKLADLGDIIGIKGTIFRTNTGELSVKATEFTLLSKSLRPLPDKYHGLKDVEQR
YRQRYLDLITNEESQNRFVMRSKILKYTRDYMDNQGFLEVETPVLHTIAGGAAAKPFITHHNALDMELYLRIALELHLKR
LIVGGMDKVYEIGRVFRNEGTSTRHNPEFTMLESYAAYEDYEDVMDLVEGLVSTVCKQVNGTTEITYGEYNVDLTPNWRR
IHMADAVKEYVGVDFWNVTSDEEARELAKKHNVPVTEHMTYGHILNEFFETYVEEKLIQPTFVYGHPVEISPLAKKNKED
DRFTDRFELFIVGREHANAFSELNDPIDQRERFEAQMKEREQGNDEAHGMDADFLEALEYGLPPTGGLGIGIDRLVMLLT
DAPSIRDILLFPTMKHRD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-105 48

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_012850.1 lysyl-tRNA synthetase VFG1668 Protein 1e-105 48