Gene Information

Name : cadA (SAR0723)
Accession : YP_040151.1
Strain : Staphylococcus aureus MRSA252
Genome accession: NC_002952
Putative virulence/resistance : Resistance
Product : cadmium-transporting ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : 3.6.3.3
Position : 760452 - 762632 bp
Length : 2181 bp
Strand : -
Note : Similar to Staphylococcus aureus plasmid pI258 probable cadmium-transporting ATPase CadA SW:CADA_STAAU (P20021) (727 aa) fasta scores: E(): 0, 91.586% id in 725 aa, and to Bacillus firmus probable cadmium-transporting ATPase CadA SW:CADA_BACFI (P30336) (7

DNA sequence :
TTGGATAGTTCAGCAAAAACATTAACAGAAGATAAACAGGTTTACCGTGTGGAGGGTTTCTCGTGTGCGAATTGTGCTGG
GAAGTTTGAAAAAAATGTAAAACAACTAGCTGGAGTTCAGGATGCAAAAGTGAACTTTGGCGCTTCCAAAATTGATGTAT
ATGGAAATGCATCGGTTCAAGAGCTTGAAAAAGCAGGTGCTTTCGAGAATCTTAAGGTATTTCCCGAAAAACTAGCGAAT
TCATCGATGCAAGCGGTCAAAGAAGACACTAAGGCTCCTAAAGAAGAGAAAATACCGTTTTATAAAAAACACAGCACATT
GCTGTTTGCCACATTACTGATTGCTTTTGGTTACCTTTCTCACTTTGTAAATGGAGAAGATAACCTTGTAACTTCCATGT
TATTTGTAGGTTCGATTGTAATTGGCGGATATTCACTATTTAAAGTTGGTTTTCAAAATTTGATACGCTTTGATTTCGAC
ATGAAAACCCTGATGACCGTAGCAGTTATTGGAGCTGCCATCATTGGTGAATGGGCAGAGGCATCCATTGTTGTCGTTCT
CTTTGCAATCAGTGAAGCACTTGAACGTTTTTCTATGGATAGAGCAAGACAGTCCATTCGTTCATTGATGGATATTGCCC
CAAAAGAAGCACTTGTTATGCGGAATGGTCAGGAAATAATGATCCATGTGGACGATATTGCCGTGGGTGATATTATGATC
GTCAAACCAGGGGAGAAAATTGCCATGGATGGGATCATTATAAATGGTGTGTCGGCTGTCAACCAGGCTGCTATAACAGG
AGAATCTGTCCCTGTTGCCAAAACGGTAGATGATGAAGTATTTGCAGGTACGCTTAACGAAGAGGGACTACTTGAAGTAA
AAATCACCAAATACGTAGAGGATACAACTATCTCCAAGATTATTCATCTGGTTGAGGAAGCACAAGGGGAGCGCGCTCCA
GCGCAAGCATTCGTAGATAAATTTGCGAAATATTATACGCCGATCATTATGGTTATTGCGGCGCTCGTTGCAGTCGTTCC
ACCTTTATTCTTTGGTGGAAGTTGGGATACTTGGGTTTATCAAGGATTAGCGGTACTTGTAGTTGGATGTCCGTGTGCAT
TAGTTATTTCTACTCCAATCTCGATTGTCTCGGCAATTGGAAATGCAGCTAAAAAAGGTGTGTTGATTAAAGGCGGTGTC
TATCTAGAGGAATTAGGAGCCATTAAGGCAATCGCATTTGATAAAACAGGAACACTGACAAAAGGTGTACCAGTGGTAAC
AGATTTTAAAGTGTTAAATGATCAAGTGGAAGAAAAAGAGCTGTTTTCCATTATTACAGCTTTAGAATATCGATCACAAC
ATCCACTTGCTTCAGCAATAATGAAGAAAGCAGAGCAAGATAATATTACTTATTCCGATGTTAGAGTGGAGGACTTCACT
TCTATTACAGGTCGGGGCATTCAAGGGAATATAGATGGAACAACCTATTACATTGGCAGTCCAAGGCTTTTTAAAGAATT
AAATGTTTCCGATTTTAGCCTTGAGTTTGAAAATAAAGTGAAAGTTTTACAAAACCAAGGGAAAACGGCCATGATTATTG
GAACGGACCAAACAATCCTCGGCGTGATTGCTGTAGCAGATGAGGTCCGCGAAACAAGTAAAAATGTGATTCAAAAACTT
CATCAGTTAGGAATCAAGCAAACAATTATGCTGACAGGTGATAATCAAGGTACCGCAGAAGCAATCGGTGCTCATGTAGG
CGTTTCTGATATTCAGTCCGAATTGATGCCACAGGATAAGTTGGACTATATTAAAAAAATGAAAGCCGAGCATGGTAATG
TAGCTATGATTGGCGATGGCGTCAATGATGCTCCTGCACTTGCTGCATCCACTGTTGGCATTGCAATGGGCGGTGCTGGA
ACAGATACTGCCATCGAGACAGCTGATATTGCATTAATGGGAGATGATTTAAGTAAGCTTCCATTTGCAGTAAGACTTAG
CAGGAAAACGCTAAATATCATCAAAGCGAACATCACGTTTGCCATCGGAATTAAGATAATTGCCTTACTATTGGTTATTC
CAGGCTGGCTAACCCTTTGGATTGCGATTCTTTCCGATATGGGAGCCACTATTTTGGTAGCATTAAATAGTTTGCGACTG
ATGAGAGTGAAGGATAAATAG

Protein sequence :
MDSSAKTLTEDKQVYRVEGFSCANCAGKFEKNVKQLAGVQDAKVNFGASKIDVYGNASVQELEKAGAFENLKVFPEKLAN
SSMQAVKEDTKAPKEEKIPFYKKHSTLLFATLLIAFGYLSHFVNGEDNLVTSMLFVGSIVIGGYSLFKVGFQNLIRFDFD
MKTLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRSLMDIAPKEALVMRNGQEIMIHVDDIAVGDIMI
VKPGEKIAMDGIIINGVSAVNQAAITGESVPVAKTVDDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAP
AQAFVDKFAKYYTPIIMVIAALVAVVPPLFFGGSWDTWVYQGLAVLVVGCPCALVISTPISIVSAIGNAAKKGVLIKGGV
YLEELGAIKAIAFDKTGTLTKGVPVVTDFKVLNDQVEEKELFSIITALEYRSQHPLASAIMKKAEQDNITYSDVRVEDFT
SITGRGIQGNIDGTTYYIGSPRLFKELNVSDFSLEFENKVKVLQNQGKTAMIIGTDQTILGVIAVADEVRETSKNVIQKL
HQLGIKQTIMLTGDNQGTAEAIGAHVGVSDIQSELMPQDKLDYIKKMKAEHGNVAMIGDGVNDAPALAASTVGIAMGGAG
TDTAIETADIALMGDDLSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILVALNSLRL
MRVKDK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 0.0 99
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 0.0 99
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 0.0 82
ORF C97 AAN62190.1 putative metal-transporting P-type ATPase Not tested PAGI-2(C) Protein 5e-124 43

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
cadA YP_040151.1 cadmium-transporting ATPase BAC0298 Protein 5e-122 48
cadA YP_040151.1 cadmium-transporting ATPase BAC0092 Protein 2e-98 43