Gene Information

Name : cadA-2 (PP_5139)
Accession : NP_747240.1
Strain : Pseudomonas putida KT2440
Genome accession: NC_002947
Putative virulence/resistance : Resistance
Product : heavy metal translocating P-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : -
Position : 5863889 - 5866141 bp
Length : 2253 bp
Strand : -
Note : similar to GP:9949854, GB:L14542, SP:Q07444, and PID:292361; identified by sequence similarity

DNA sequence :
ATGAACCAGCCTGTCAGCCACGAACACAAGCATCCGCACGATCACGCTCATGGTGATGACGACCATGGCCATGCTGCCCA
CGGCCACAGCTGCTGCGGCGCCAAAGCCGCGCCGCCGCTGGTGCAACTGAGCGAAACGGCCAGTGCCCAGGCACAACTCA
GTCGTTTCCGCATCGAGGCCATGGACTGCCCCACCGAGCAGACCCTGATCCAGGACAAACTGAGCAAGCTGGCCGGTATC
GAACAGCTGGAATTCAACCTGATCAACCGCGTGCTTGGCGTGCGCCACACCCTGGACGGCACCGCCGATATCGAGCGGGC
GATCGACAGCCTGGGCATGAAGGCCGAGCCGATAGCGGCCCAGGACGACGGCAGTGCCAGCGTGCCACAACCGGCCAAGG
CCCACTGGTGGCCACTGGCATTGTCGGGCGTCGCCGCGATCGCCGCCGAAATCGTGCACTTTGCTGCACTGGCCCCTGAG
TGGGTGGTGGCGGGGCTGGCGCTTGCCGCGATCCTCGGTTGCGGCCTGGGTACCTACAAGAAGGGCTGGATTGCCCTGAA
AAACCGGAACCTCAACATCAATGCGCTGATGAGTATCGCCGTGACGGGGGCCGTATTGATCGGCCAGTGGCCGGAAGCGG
CTATGGTGATGGTGCTGTTCACCGTTGCCGAACTGATCGAAGCGCGCTCGCTGGACCGTGCGCGCAATGCCATTGGCGGG
TTGATGCAGCTGACCCCGGACATGGCCACGGTGCAGCAGGCCGATGGCCAGTGGCGCGAGCTGGACGTGCGTGAGGTGGC
CATCGGCGCACTGGTGCGGGTGCGCCCCGGTGAGCGCATCGGACTGGACGGTGAAGTGACCCGCGGGCAATCCAGCGTCG
ATCAGGCGCCGATTACCGGTGAAAGCCTGCCTGTGGAGAAGGGGGTGGGTGACAAGCTGTTCGCTGGCACCATCAACCAG
GCGGGGGCGCTGGAGTTTCGCGTCACCGCCGCTGCCGGGCAGTCGACCCTGGCGCGGATCATCAAGGCGGTGGAGGAAGC
GCAAGGCGCGCGGGCGCCTACCCAGCGCTTTGTCGACCGTTTTTCGCGCATCTACACCCCGGTGGTGTTCGCCATCGCCT
TGGCCGTGGCGCTGATACCACCGCTGTTCCTGGCTGGCGCCTGGTTCGACTGGGTTTACCGCGCCTTGGTGCTGCTGGTG
GTGGCCTGCCCGTGCGCGCTGGTGATCTCGACCCCGGTGACCATCGTCAGTGGCCTGGCCGCCGCCGCGCGCAAAGGCAT
CCTGATCAAGGGTGGCGTGTACCTGGAGGGTGGCCGCCACCTTGATTTCCTGGCCCTGGACAAGACCGGCACCATCACCC
ATGGCAAGCCGGTGCAGACCGACGCCAAGGTGCTGGCGCCGCTGTTCGAAGACCGTGCGCAAGCCTTGGCGGCCAGCCTG
GGCGAGCGTTCGGACCACCCGGTGTCCCGCGCCATTGCCGAGTTCGGCAAGCAGCAAGGCCTGGCCTTGAGCGAGGTCAG
TGACTTCGTTGCGCTGGCCGGGCGTGGCGTGCGTGGCGTCATTGCCGGTGAGGTCTACCACTTGGGCAACCACCGCCTGG
TCGAGGAGCTGGGGCTGTGTTCGCCAGCGCTGGAGGCCCAACTGGATGCGCTGGAGCGCCAGGGCAAGACCGTGGTCCTG
CTGCTTGACCGCTCCGGGCCACTGGCACTGTTCGCCGTGGCCGACACGGTCAAGGACAGCAGCCGTCAGGCGATCGCCGA
ATTGCATCAGCTGGGTATCAAGACTGTCATGCTGACCGGTGACAATCCGCATACTGCGCAGGCCATTGCTGCCGTGGTGG
GAATCGACCGCGCCGAGGGCAACCTGTTGCCGGCCGACAAGCTGAAAACCATTGAGGCGTTGTACGCACAAGGCCATCGG
GTGGGCATGGTCGGTGACGGCATCAACGACGCCCCGGCGCTGGCCCGCGCCGAAATCGGTTTTGCCATGGCTGCTGCCGG
CACCGACACCGCCATCGAGACGGCCGATGTGGCGCTGATGGACGATGACTTGCGCAAAATCCCGGCCTTCGTCAGGCTGT
CGCGCCAAAGTGCGGCGATCCTCATGCAGAACATCGTGCTGGCGTTGGGCATCAAGGCGATATTTCTGGCGATCACCTTC
GCCGGCATGGCCACGATGTGGATGGCGGTGTTCGCTGATATGGGCGTGAGCTTGCTGGTGGTGTTCAACGGCTTGCGCCT
TTTGCGCAAATAG

Protein sequence :
MNQPVSHEHKHPHDHAHGDDDHGHAAHGHSCCGAKAAPPLVQLSETASAQAQLSRFRIEAMDCPTEQTLIQDKLSKLAGI
EQLEFNLINRVLGVRHTLDGTADIERAIDSLGMKAEPIAAQDDGSASVPQPAKAHWWPLALSGVAAIAAEIVHFAALAPE
WVVAGLALAAILGCGLGTYKKGWIALKNRNLNINALMSIAVTGAVLIGQWPEAAMVMVLFTVAELIEARSLDRARNAIGG
LMQLTPDMATVQQADGQWRELDVREVAIGALVRVRPGERIGLDGEVTRGQSSVDQAPITGESLPVEKGVGDKLFAGTINQ
AGALEFRVTAAAGQSTLARIIKAVEEAQGARAPTQRFVDRFSRIYTPVVFAIALAVALIPPLFLAGAWFDWVYRALVLLV
VACPCALVISTPVTIVSGLAAAARKGILIKGGVYLEGGRHLDFLALDKTGTITHGKPVQTDAKVLAPLFEDRAQALAASL
GERSDHPVSRAIAEFGKQQGLALSEVSDFVALAGRGVRGVIAGEVYHLGNHRLVEELGLCSPALEAQLDALERQGKTVVL
LLDRSGPLALFAVADTVKDSSRQAIAELHQLGIKTVMLTGDNPHTAQAIAAVVGIDRAEGNLLPADKLKTIEALYAQGHR
VGMVGDGINDAPALARAEIGFAMAAAGTDTAIETADVALMDDDLRKIPAFVRLSRQSAAILMQNIVLALGIKAIFLAITF
AGMATMWMAVFADMGVSLLVVFNGLRLLRK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ORF C97 AAN62190.1 putative metal-transporting P-type ATPase Not tested PAGI-2(C) Protein 0.0 67
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 1e-109 48
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 1e-109 48
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 2e-107 47

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
cadA-2 NP_747240.1 heavy metal translocating P-type ATPase BAC0298 Protein 0.0 67
cadA-2 NP_747240.1 heavy metal translocating P-type ATPase BAC0455 Protein 2e-78 43
cadA-2 NP_747240.1 heavy metal translocating P-type ATPase BAC0461 Protein 1e-77 42
cadA-2 NP_747240.1 heavy metal translocating P-type ATPase BAC0092 Protein 2e-72 41