Gene Information

Name : PP_4420 (PP_4420)
Accession : NP_746533.1
Strain : Pseudomonas putida KT2440
Genome accession: NC_002947
Putative virulence/resistance : Unknown
Product : transposase, OrfB
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG2801
EC number : -
Position : 5014541 - 5015350 bp
Length : 810 bp
Strand : +
Note : identified by match to PFAM protein family HMM PF00665

DNA sequence :
GTGTTCGAAGTCACTCGCTCAAGTTACTACGCCCATCGTCATAGAAGACGAACTCCGGACGTCGAGCGACTGCGATTGCG
CAGTCGAGTGAATGAGTTGTTCGCGCGAGGGCGAAGTGCGCCCGGCAGCCGTAGCGTCAAGGCGATGATGCAAGAGGAAG
GTGAGCAAATCGGGCGGTTCAAGGTGCGCAGCCTGATGCGTGAGCTAGCGTTAGTCAGCAAGCAGCCAGGGGCCTATGCG
TACAAAAAGGCCACAGTGGAGCGGCCAGATATTCCGAATATTTTGAACCGGGCGTTTGATGTCGAAGCGCCAAATCAGGT
CTGGTGTGGCGACATCACTTACATTTGGGCGCAGGGCAAATGGCACTACTTGGCAGTTGTCCTGGATCTTTATGCACGCA
GAGTGGTGGGCTGGGCCCTATCAGAGAAGCCGGACGCAGACCTCGTGGTCAAGGCGCTGGACGTGGCTTACGAACAACGT
GGCAAACCTCAAGGGCTACTGTTCCACTCAGACCAGGGGTCGCAATATGGCAGCCGTCAGTTCCGCCAGAGACTGTGGCG
CTACCGCATGCGCCAGAGTATGAGCCGCCGAGGTAACTGCTGGGATAATGCGCCGATGGAGCGAGTATTTCGTAGCTTGA
AATCGGAGTGGATACCGACCACTGGATACCTGACTGGGCAGCAAGCTCAACGAGACATCAGCCAATACCTGATGAGGCAC
TACAACTGGATCCGCCCGCATCAATTCAACAATGGGCTGGCTCCGGCAAAAGCTGAAGAAAAACTCAAAACTGTGTCCGG
AATGAGTTGA

Protein sequence :
MFEVTRSSYYAHRHRRRTPDVERLRLRSRVNELFARGRSAPGSRSVKAMMQEEGEQIGRFKVRSLMRELALVSKQPGAYA
YKKATVERPDIPNILNRAFDVEAPNQVWCGDITYIWAQGKWHYLAVVLDLYARRVVGWALSEKPDADLVVKALDVAYEQR
GKPQGLLFHSDQGSQYGSRQFRQRLWRYRMRQSMSRRGNCWDNAPMERVFRSLKSEWIPTTGYLTGQQAQRDISQYLMRH
YNWIRPHQFNNGLAPAKAEEKLKTVSGMS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
RS04 AAP82949.1 putative transposase Not tested PAPI-2 Protein 6e-91 75
l7094 CAD33743.1 ORF B protein Not tested PAI I 536 Protein 3e-59 58
orfB CAE85178.1 OrfB protein, IS911 Not tested PAI V 536 Protein 3e-59 58
unnamed AAC31485.1 L0006 Not tested LEE Protein 6e-60 53
ECs4537 NP_312564.1 hypothetical protein Not tested LEE Protein 8e-60 53
unnamed CAI43898.1 putative transposase Not tested LEE Protein 3e-61 53
aec65 AAW51748.1 Aec65 Not tested AGI-3 Protein 3e-61 53
Z5089 NP_290241.1 transposase Not tested LEE Protein 1e-61 52
unnamed ACU09432.1 IS911 transposase orfB Not tested LEE Protein 1e-61 52
ECO111_3779 YP_003236114.1 putative IS602 transposase OrfB Not tested LEE Protein 1e-63 51
orfB AGK06910.1 IS1359 transposase; OrfB Not tested SGI1 Protein 2e-65 51
overlap with orfA characteristic of IS3 family members AGK06947.1 IS1359 transposase; OrfB Not tested SGI1 Protein 2e-65 51
orfB AGK06993.1 IS1359 transposase; OrfB Not tested SGI1 Protein 2e-65 51
orfB AGK07051.1 IS1359 transposase; OrfB Not tested SGI1 Protein 2e-65 51
VC1789 NP_231424.1 transposase OrfAB subunit B Not tested VPI-2 Protein 3e-65 51
orfB ACX47958.1 IS1359 transposase; OrfB Not tested SGI1 Protein 2e-65 51
orfB YP_001217329.1 transposase OrfAB subunit B Not tested VPI-2 Protein 3e-65 51
VPI2_0008c ACA01825.1 transposase OrfAB subunit B Not tested VPI-2 Protein 2e-65 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
PP_4420 NP_746533.1 transposase, OrfB VFG1484 Protein 2e-59 58
PP_4420 NP_746533.1 transposase, OrfB VFG0785 Protein 6e-62 52
PP_4420 NP_746533.1 transposase, OrfB VFG1122 Protein 1e-65 51