Gene Information

Name : lysS (DET0578)
Accession : YP_181320.1
Strain : Dehalococcoides ethenogenes 195
Genome accession: NC_002936
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 530455 - 531951 bp
Length : 1497 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGCCGGAAGAATATCTGAACGCCCAAAAGATGGAAAAACTGGAACGGATAAAGAGCCGGGGTATAAACCCCTATCCAGC
CACTTTCCACCCCAGCCATACCAGTGTCCAGGCAGTTGCCTTGCTGGCCGAACTGGAAAAACAGGAGAACCCGCCAAAGG
AAATTCTGAGGATTGCCGGGCGGATTATGACCCTGCGCGACATGGGCAAGATTTCTTTCATGGATATACGTGACGGCAGC
GGCAAAATACAGGTTTTCTGCCGCCAGAATGACCTTGACGAAGCCAGTATAGAGCTGCTTAAAGATTTGGATTTGGGTGA
TTTTATAGGTGCGGAGGGAAGCCTTATGCGTACCCGCACCGGTGAACCCAGTCTGGCAGCCACCAAAATAAGCCTGCTTT
CCAAATCCCTGCTGCCCCTGCCTGAGAAATGGCACGGCCTGCAGGATGTGGAAAAACGCTACCGCCAGCGTTACCTGGAC
CTTATTTCCAATGCGGACGCCCGCCAGACCTTCCTTACCCGCAGCCAGGTTATCAGTGCCATCCGCAGCTTTATGAGCAG
CAAAGGTTTTCTGGAGGTAGAAACCCCGGTTCTTCAGCCTGAGGCAGGCGGGGCACTCGCCCGCCCCTTTATTACCCATC
ATCAGGCTTTGGACTGTGATTTTTATATGCGGATAGCTTTGGAACTGCACCTGAAAAGGCTGATTGTGGGCGGCTTTGAC
CGGGTATATGAGATAGGGCGGATATTCCGCAACGAAGGGGTTTCCACCCGCCACAACCCCGAATTTACCATGATGGAAAG
CTACCAGGCTTATGCTGACTATAAAGACGTTATGGATTTCCTTGAGGAAATGGTTTCAAGTGTGGTTAAAGAAATAAGCG
GCGGATACACCGTGCCATTCGGGGATACTACACTGGATTTTACCCCTCCCTGGCCCCGCCTGAGCATGCGTGATGCCGTA
AAGCAATATGCCGGTATAGATTTCTTTGATTTCCCCACCAAAGAGGCTTTAGCCGCAGAGATGATTCGCCGCAAACTGAA
GGTAGACCCCGCCAAAGACTGGGGCAAACTGGTTGATGAGCTGGTGGGTGAATTTGTAGAACCCCGTTTGATTCAACCCA
CTTTCCTTACTGACCACCCGGTGGCCATGTCCCCGCTTGCCAAACAGAAGCCGGAAGACCCCCGCCTGACAGAGCGGTTT
GAAGCCATTTGTGCCAATATGGAAATAGCCAATGCCTTTTCCGAACTGAATGACCCGGTAGAGCAAAGGGCGCGTTTTAA
GGAACAGCTGGAAAAGCGCAATCAACTGCGTACGGAAGAGTCCGAAAGTATAGACGAGGACTTTTTGGCCGCTCTGGCAT
ATGGTATGCCGCCCACCGGCGGTCTGGGTGTAGGTATTGACCGTCTGGTAATGCTGCTTACCAACCATGACTCTATCCGT
GAGGTTATTTTGTTCCCTGCCCTGAAAGACCGGGAAGACAAGGGCGAACAGGAATAA

Protein sequence :
MPEEYLNAQKMEKLERIKSRGINPYPATFHPSHTSVQAVALLAELEKQENPPKEILRIAGRIMTLRDMGKISFMDIRDGS
GKIQVFCRQNDLDEASIELLKDLDLGDFIGAEGSLMRTRTGEPSLAATKISLLSKSLLPLPEKWHGLQDVEKRYRQRYLD
LISNADARQTFLTRSQVISAIRSFMSSKGFLEVETPVLQPEAGGALARPFITHHQALDCDFYMRIALELHLKRLIVGGFD
RVYEIGRIFRNEGVSTRHNPEFTMMESYQAYADYKDVMDFLEEMVSSVVKEISGGYTVPFGDTTLDFTPPWPRLSMRDAV
KQYAGIDFFDFPTKEALAAEMIRRKLKVDPAKDWGKLVDELVGEFVEPRLIQPTFLTDHPVAMSPLAKQKPEDPRLTERF
EAICANMEIANAFSELNDPVEQRARFKEQLEKRNQLRTEESESIDEDFLAALAYGMPPTGGLGVGIDRLVMLLTNHDSIR
EVILFPALKDREDKGEQE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-103 48

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_181320.1 lysyl-tRNA synthetase VFG1668 Protein 1e-103 48