Gene Information

Name : ECs0148 (ECs0148)
Accession : NP_308175.2
Strain : Escherichia coli Sakai
Genome accession: NC_002695
Putative virulence/resistance : Unknown
Product : glutamyl-Q tRNA(Asp) synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG0008
EC number : -
Position : 163508 - 164404 bp
Length : 897 bp
Strand : -
Note : this tRNA synthetase lacks the tRNA anticodon interaction domain; instead this enzyme modifies tRNA(Asp) with glutamate by esterifying glutamate to the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of queosine generating a modified nucleoside at the

DNA sequence :
ATGACAGACACACAGTATATTGGCCGCTTCGCCCCCTCTCCTTCCGGCGAGCTTCATTTTGGTTCTCTGATTGCTGCGCT
TGGCAGCTATTTGCAGGCTCGCGCCCGGCACGGTCGCTGGCTGGTACGCATTGAAGATATCGACCCGCCTCGTGAAGTTC
CCGGTGCCGCAGAAACTATCCTGCGCCAGCTGGAACATTACGGTCTGCACTGGGACGGCGATGTTCTCTGGCAATCGCAA
CGTCACCACGCCTATCGTGAAGCACTCGCCTGGTTACATGAACAAGGACTAAGTTATTACTGCACCTGCACGCGTGCGCG
TATTCAAAGCATTGGCGGTATTTACGACGGTCATTGCCGGGTGTTGCATCATGGACCAGACAACGCCGCAGTGCGTATCC
GCCAGCAGCATCCGGTCACGCAATTTACTGACCAGCTGCGCGGCATTATTCACGCCGACGAAAAACTGGCACGGGAAGAT
TTTATTATTCATCGCCGTGATGGTTTGTTTGCCTATAACCTGGCGGTGGTGGTGGATGATCACTTCCAGGGCGTGAGCGA
AATTGTCCGCGGGGCAGATTTGATCGAACCGACAGTGCGGCAAATCTCGCTGTACCAGCTTTTTGGCTGGAAAGTGCCAG
ATTACATTCATCTGCCGCTGGCGCTTAATCCACAAGGCGCTAAACTTTCCAAGCAGAATCATGCGCCTGCGTTGCCGAAA
GGCGATCCACGCCCGGTACTAATCGCGGCACTTCAATTTCTGGGGCAGCAGGCAGAAGCACACTGGCAGGATTTCAGCGT
CGAGCAAATCCTTCAGTCAGCCGTCAAAAACTGGCGGCTTACCGCAGTGCCTGAGTCGGCAATTGTAAATTCAACATTCT
CAAATGCGTCATGCTGA

Protein sequence :
MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQ
RHHAYREALAWLHEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHLPLALNPQGAKLSKQNHAPALPK
GDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVKNWRLTAVPESAIVNSTFSNASC

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
BJAB07104_00313 YP_008207778.1 Glutamyl- and glutaminyl-tRNA synthetase Not tested AbaR25 Protein 2e-54 44
BJAB0868_00317 YP_008211643.1 Glutamyl- and glutaminyl-tRNA synthetase Not tested AbaR26 Protein 2e-54 44