Gene Information

Name : ddl (mll1812)
Accession : NP_103309.1
Strain : Mesorhizobium loti MAFF303099
Genome accession: NC_002678
Putative virulence/resistance : Resistance
Product : D-alanyl-alanine synthetase A
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : -
Position : 1500630 - 1501703 bp
Length : 1074 bp
Strand : -
Note : D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli

DNA sequence :
ATGAAGGAAAAAACAAGGGTCGCCATCCTCTATGGCGGGCGTTCGGCCGAGCATGACGTGTCGCGGCTGTCGGCCGCCAA
TGTGCTGAAGGCGATCGACCGGACGCGCTACGAGGTGGTGCCGATCGCCATTTCCAGGGACGGCAGATGGCGGCTGCAGC
CTTCGTCCGAGGCTGGCGCCGACGGGGCAGGGGCTCAGGTGCCGGAGGATGGCGTGGAGGTCGCGCTGCTGCCCGGCGGC
AAGGGCCGGCTGGTGGCTCTCACCAAGAATGGCGAGCAGCCCGACCCTGTCGATGTCGTCTTTCCCGTGCTGCATGGGCC
GTTCGGCGAGGACGGTTCGGTGCAAGGCTATGCCGAGGTTGCCGACGTCGCCTATGTCGGCTGCGGCATCCTGGCTTCGG
CGGCTGCCATGGACAAGGATGTGGCCAAGCGGCTGCTGCGCGAGGCGGGGCTTGCCGTGGCCCGCTCGGTCACCGTGCTG
AAAGGCGACGTCGGTTCGTTCCAGGAAATTGCCGGGGCGCTCGGGCTGCCCTTCTTCGCCAAGCCGGCGCGCCAGGGCTC
GTCCTTCGGGGTGAGCAAGGTGCATGACAGGGATGGTTTCGAGCAGGCCGTCGAAACGGCTTTGCGCTATGACAGCAAGG
CGCTGATCGAGGAGTTCGTCGACGGCCGCGAGATCGAGTGCTCGGTGCTGGAGCGGGCCGACGGTTCGCTGACGGTTTCG
CCGCCAGGCGAGATCATTCCGGCCGACAAGCATGGGTTCTACACCTACGAAGCGAAGTATTTCGACGCCGAAGGCGCGGT
GGTGAAGGTGCCCGCCGATGTCCCGGCGGATGTTGTTGCCAGGACGAGCGAGATGGCCGCGCGGGCTTTCCGGGCGCTCG
GCTGCGAGGCCATGGCGCGCGTCGACTTCTTCCTGCGCGCCGACGGTTCGCTGCTGGTCAACGAAGTCAACACGCTGCCC
GGCTTCACCGACATCTCGATGTACGCCAAGGCGCTGGCGGCGGCCGGCATCGGCTATAGCAAGGTGATCGACGTGCTGAT
CGAGCACGCGCTGGCAAGGCACCGGGCGCGCTGA

Protein sequence :
MKEKTRVAILYGGRSAEHDVSRLSAANVLKAIDRTRYEVVPIAISRDGRWRLQPSSEAGADGAGAQVPEDGVEVALLPGG
KGRLVALTKNGEQPDPVDVVFPVLHGPFGEDGSVQGYAEVADVAYVGCGILASAAAMDKDVAKRLLREAGLAVARSVTVL
KGDVGSFQEIAGALGLPFFAKPARQGSSFGVSKVHDRDGFEQAVETALRYDSKALIEEFVDGREIECSVLERADGSLTVS
PPGEIIPADKHGFYTYEAKYFDAEGAVVKVPADVPADVVARTSEMAARAFRALGCEAMARVDFFLRADGSLLVNEVNTLP
GFTDISMYAKALAAAGIGYSKVIDVLIEHALARHRAR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 1e-49 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
ddl NP_103309.1 D-alanyl-alanine synthetase A AY145441.gene.p01 Protein 1e-48 41
ddl NP_103309.1 D-alanyl-alanine synthetase A AF310956.2.orf5.gene Protein 7e-49 41