Gene Information

Name : metB2 (L0181)
Accession : NP_266937.1
Strain : Lactococcus lactis IL1403
Genome accession: NC_002662
Putative virulence/resistance : Resistance
Product : cystathionine gamma-synthase
Function : -
COG functional category : E : Amino acid transport and metabolism
COG ID : COG0626
EC number : 2.5.1.48
Position : 790537 - 791679 bp
Length : 1143 bp
Strand : +
Note : catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine and L-cysteine; or Cystathionine beta-lyase (CBL) can convert cystathionine to homocysteine

DNA sequence :
ATGACAAGTATAAAAACGAAAGTAATTCATGGTGGTATTTCAACAGACAAGACAACAGGAGCAGTATCTGTTCCGATTTA
TCAAACGTCAACTTATAAACAAAACGGACTTGGACAACCTAAAGAATACGAATATTCACGTTCTGGAAATCCAACGCGTC
ATGCCTTGGAAGAATTAATTGCAGATTTAGAAGGTGGTGTTCAAGGATTTGCATTTAGTTCAGGACTTGCTGGAATTCAT
GCGGTTCTTTCGCTATTTTCTGCAGGAGATCATATCATTTTAGCTGATGATGTTTATGGTGGAACTTTCCGTTTGATGGA
TAAAGTGCTGACAAAGACAGGGATTATTTATGATTTAGTTGATTTGAGTAATCTTGATGACTTGAAAGCGGCTTTTAAAG
AAGAAACGAAAGCTATTTATTTTGAAACTCCTTCAAATCCTTTATTAAAAGTTTTGGACATTAAGGAAATTTCAGCAATT
GCTAAAGCTCATGATGCCTTAACACTGGTGGATAATACCTTTGCGACACCGTATTTACAACAACCAATTGCTTTAGGAGC
TGATATTGTTTTGCATTCCGCAACGAAATATTTAGGTGGACATTCAGATGTAGTGGCAGGACTAGTTACAACCAATTCAA
AAGAACTCGCCAGCGAAATTGGCTTTTTACAAAATTCAATTGGGGCGGTTCTTGGACCACAAGACAGCTGGTTAGTGCAA
AGAGGTATTAAAACACTTGCGCTTCGGATGGAGGCTCATTCGGCAAATGCTCAAAAAATTGCTGAGTTTTTAGAAACTTC
TAAAGCCGTCTCAAAAGTTTATTATCCAGGTCTTAATAGCCATCCGGGGCATGAAATTGCTAAAAAACAAATGTCTGCCT
TTGGAGGGATGATTTCATTTGAATTAACGGATGAAAATGCCGTCAAAGATTTTGTTGAAAACTTGAGCTATTTTACGTTG
GCTGAATCACTAGGTGGCGTTGAATCTTTAATTGAAGTGCCAGCAGTGATGACTCATGCTTCTATTCCAAAAGAACTTCG
TGAAGAAATTGGGATTAAGGATGGCCTAATTCGTCTATCAGTAGGGGTTGAAGCAATTGAAGATTTACTGACAGATATCA
AAGAAGCACTTGAAAAAAAATAA

Protein sequence :
MTSIKTKVIHGGISTDKTTGAVSVPIYQTSTYKQNGLGQPKEYEYSRSGNPTRHALEELIADLEGGVQGFAFSSGLAGIH
AVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIYDLVDLSNLDDLKAAFKEETKAIYFETPSNPLLKVLDIKEISAI
AKAHDALTLVDNTFATPYLQQPIALGADIVLHSATKYLGGHSDVVAGLVTTNSKELASEIGFLQNSIGAVLGPQDSWLVQ
RGIKTLALRMEAHSANAQKIAEFLETSKAVSKVYYPGLNSHPGHEIAKKQMSAFGGMISFELTDENAVKDFVENLSYFTL
AESLGGVESLIEVPAVMTHASIPKELREEIGIKDGLIRLSVGVEAIEDLLTDIKEALEKK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SAR0460 YP_039909.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 4e-97 55
SAS0418 YP_042545.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 2e-98 55
metB YP_281510.1 cystathionine beta-lyase Not tested Not named Protein 2e-74 46

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
metB2 NP_266937.1 cystathionine gamma-synthase BAC0207 Protein 5e-72 44