Gene Information

Name : pyk (L0003)
Accession : NP_267488.1
Strain : Lactococcus lactis IL1403
Genome accession: NC_002662
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 1370319 - 1371827 bp
Length : 1509 bp
Strand : -
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGAATAAACGTGTAAAAATCGTCTCAACTCTTGGACCTGCTGTCGAAATTCGTGGTGGCAAAAAATTTGGTGAAAGCGG
CTATTGGGGAGAATCTCTTGACGTCGAAGCATCAGCAAAAAACATTGCAGCTTTGATTGAAGAAGGCGCAAATGTCTTCC
GTTTCAACTTCTCACACGGTGACCATCCTGAACAAGGTGCTCGTATGGCAACAGTTCACCGCGCTGAAGAAATCGCTGGT
CATAAAGTAGGTTTCTTGCTTGATACTAAAGGTCCTGAAATGCGTACAGAACTCTTTGCTGACGGTGCTGATGCAATCAG
CGTTGTTACTGGAGATAAATTCCGCGTTGCAACTAAACAAGGTCTTAAATCAACTCCTGAATTGATTGCTTTGAACGTAG
CTGGTGGTCTTGATATCTTTGATGATGTTGAAATCGGTCAAACTATTTTGATTGACGATGGTAAACTTGGTTTGTCATTG
ACTGGTAAAGATGCAGCAACTCGTGAGTTTGAAGTTGAAGCACAAAATGATGGCGTTATTGGTAAACAAAAAGGTGTTAA
CATTCCTAACACTAAAATTCCTTTCCCAGCTCTTGCTGAACGTGATGATGCGGATATCCGTTTCGGACTTTCACAACCTG
GTGGAATCAACTTTATCGCTATCTCATTCGTACGTACTGCAAATGACGTTAAAGAAGTACGTCGTATTTGTGAAGAAACT
GGAAATCCACACGTACAACTTTTGGCTAAAATTGAAAACCAACAAGGTATCGAAAACCTTGATGAAATCATCGAAGCAGC
TGATGGTATCATGATTGCTCGTGGTGACATGGGTATCGAAGTTCCATTTGAAATGGTTCCAGTTTACCAAAAATTGATCA
TCAGCAAAGTTAACAAAGCAGGTAAAATCGTTGTTACTGCAACAAACATGCTTGAATCAATGACTTACAACCCACGTGCT
ACTCGTTCAGAAATTTCAGACGTATTCAACGCTGTTATTGACGGAACTGACGCAACAATGCTTTCAGGTGAATCTGCTAA
TGGTAAATACCCACGCGAATCAGTTCGTACAATGGCTACTGTTAACAAAAATGCTCAAACAATGTTGAAAGAATACGGAC
GTCTTCACCCAGAACGTTACGACAAATCAACTGTAACAGAAGTAGTTGCTGCATCAGTTAAAAACGCTGCAGAAGCTATG
GATATCAAATTGATTGTTGCTTTGACTGAATCAGGTAACACTGCTCGTTTGATTTCTAAACACCGTCCTAATGCTGATAT
CTTGGCTATTACATTTGACGAAAAAGTTGAACGTGGATTGATGATTAACTGGGGTGTAATCCCAACAATGACTGAAAAAC
CTTCATCTACAGATGACATGTTTGAAGTTGCTGAAAAAGTTGCTTTGGCTTCAGGTTTAGTTGAATCTGGTGACAATATC
ATCATCGTTGCTGGTGTTCCAGTTGGTACTGGTCGTACAAATACAATGCGTATCCGCACTGTTAAATAA

Protein sequence :
MNKRVKIVSTLGPAVEIRGGKKFGESGYWGESLDVEASAKNIAALIEEGANVFRFNFSHGDHPEQGARMATVHRAEEIAG
HKVGFLLDTKGPEMRTELFADGADAISVVTGDKFRVATKQGLKSTPELIALNVAGGLDIFDDVEIGQTILIDDGKLGLSL
TGKDAATREFEVEAQNDGVIGKQKGVNIPNTKIPFPALAERDDADIRFGLSQPGGINFIAISFVRTANDVKEVRRICEET
GNPHVQLLAKIENQQGIENLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKLIISKVNKAGKIVVTATNMLESMTYNPRA
TRSEISDVFNAVIDGTDATMLSGESANGKYPRESVRTMATVNKNAQTMLKEYGRLHPERYDKSTVTEVVAASVKNAAEAM
DIKLIVALTESGNTARLISKHRPNADILAITFDEKVERGLMINWGVIPTMTEKPSSTDDMFEVAEKVALASGLVESGDNI
IIVAGVPVGTGRTNTMRIRTVK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-76 42
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 8e-77 42
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 8e-77 42
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 3e-77 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk NP_267488.1 pyruvate kinase VFG0479 Protein 4e-77 42