Gene Information

Name : fliI (BH2455)
Accession : NP_243321.1
Strain : Bacillus halodurans C-125
Genome accession: NC_002570
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 2573206 - 2574519 bp
Length : 1314 bp
Strand : -
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGGACGCTAGATCACTCATTTCTCTGATTCCATCGTTATCTGCTTATAAATGGTATGGAAAAGTGACTCGCGTGATTGG
TTTAACGATAGAAGCAAAAGGACCACAAGCTTCCATCGGTGAATTGTGTTACATCCTCGTAGGAAAACAAGGAACAAAAG
TGATGGCTGAGGTAGTCGGCTTTAAGGAAGAGCGAGTGCTTTTAATGCCGCTTAACGGGACGGCAGAGATTGCTCCTGGT
AGCTTAGTTGAAGGAACCGGTCGCCCTCTCCAAGTGAAGGTGGGCTATAGCTTAATTGGCCATGTCATTGATGGCTGTGG
TCGGCCTTTAACTGGGGAAGCATTACCTAATGGGTTAACGCCGTATCCGACAGATAACCCGCCGCCAAATCCCATTTCTC
GTCCACGGATTAAGGAAGCGATGCACTTAGGGGTTCGCGCGATAGACGGACTGCTTACAGTCGGAAAAGGACAACGTGTC
GGAATTTTTGCCGGAAGTGGCGTAGGGAAAAGCACACTCCTTGCCATGTTAGCGAAAAACGCTGCCGCTGATCTGAACAT
CATTGCTCTTATCGGCGAACGGGGACGAGAAGTGAAAGATTTTATTGAAAGGGATTTAGGCGAAGATGGACTTAGGCGGT
CGATTATCGTAGTGGCCACCTCAGACCAACCAGCCCTCATGCGGATTAAAGGGGCGATGACGGCAACTGCTCTTGCTGAA
TATTTTAGGGACCAGGGCCTACACGTCAATTTAATGATGGATTCAGTCACAAGGTTTGCGATGGCACAACGGGAAATTGG
TCTAGCGGTCGGTGAACCTCCGACAACGAAAGGCTATACTCCATCAGTTTTCGCTTTGCTACCAAAATTATTGGAACGTG
CAGGAACCAATGGGAAAGGGAGCATCACTGGCTTCTACACGGTTCTCGTCGACGGAGATGATTTGAATGAACCGATCTCA
GATACGGTTCGTGGAATTCTTGATGGGCATTTCGTCCTTGATCGCGAGCTTGCCAATAAGGGGCATTATCCGGCGATCAA
CATTTTAAAAAGTGTGAGTAGGGTGATGAATGATATTGTTACAGATGAGCAAAAGCGTGCTGCAGAGCATGTTCGCTCAC
TATTATCGGTATATAAAGAATCGGAAGACTTAATTAACATTGGCGCTTACAAGCGTGGCTCATCGAAACAAATTGATGAA
GCGATCGAGCGACAGCCGGAAGTGATTTCCTTTTTAAGACAAAATACGGATGAAGTGGCGATTGCTGAACATTCGATCCA
ACAATTAATTCAAGCTTTTGGAAAGGGTGATTGA

Protein sequence :
MDARSLISLIPSLSAYKWYGKVTRVIGLTIEAKGPQASIGELCYILVGKQGTKVMAEVVGFKEERVLLMPLNGTAEIAPG
SLVEGTGRPLQVKVGYSLIGHVIDGCGRPLTGEALPNGLTPYPTDNPPPNPISRPRIKEAMHLGVRAIDGLLTVGKGQRV
GIFAGSGVGKSTLLAMLAKNAAADLNIIALIGERGREVKDFIERDLGEDGLRRSIIVVATSDQPALMRIKGAMTATALAE
YFRDQGLHVNLMMDSVTRFAMAQREIGLAVGEPPTTKGYTPSVFALLPKLLERAGTNGKGSITGFYTVLVDGDDLNEPIS
DTVRGILDGHFVLDRELANKGHYPAINILKSVSRVMNDIVTDEQKRAAEHVRSLLSVYKESEDLINIGAYKRGSSKQIDE
AIERQPEVISFLRQNTDEVAIAEHSIQQLIQAFGKGD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-89 53
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-87 52
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-87 52
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-87 52
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-87 51
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 4e-87 51
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 5e-88 51
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-85 51
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-85 51
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-87 51
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-83 49
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 8e-79 49
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 3e-83 48
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 6e-83 48
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-83 48
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-83 48
escN CAI43874.1 EscN protein Virulence LEE Protein 3e-79 48
escN YP_003223475.1 translocator EscN Virulence LEE Protein 4e-79 48
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-73 48
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-73 48
escN YP_003236088.1 translocator EscN Virulence LEE Protein 2e-78 48
escN AAC38383.1 EscN Virulence LEE Protein 7e-79 48
escN AAC31513.1 L0034 Virulence LEE Protein 2e-78 48
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-78 48
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-78 48
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-78 48
unnamed AAL06369.1 EscN Virulence LEE Protein 3e-76 48
escN CAC81862.1 EscN protein Virulence LEE II Protein 7e-79 47
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 9e-79 47
escN AAK26715.1 EscN Virulence LEE Protein 3e-78 47
escN AAL57542.1 EscN Virulence LEE Protein 3e-78 47
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 1e-77 45
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 3e-72 45
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-72 45
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 6e-73 45
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 6e-69 45
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 2e-66 44
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-68 44
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-68 44
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-66 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 8e-68 43
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 6e-67 42
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 6e-67 42
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-64 41
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 3e-64 41
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-64 41
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-64 41
invC AAS66861.1 InvC Not tested SSR-2 Protein 1e-65 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI NP_243321.1 flagellum-specific ATP synthase VFG0192 Protein 8e-91 54
fliI NP_243321.1 flagellum-specific ATP synthase VFG0390 Protein 8e-93 53
fliI NP_243321.1 flagellum-specific ATP synthase VFG2144 Protein 1e-92 51
fliI NP_243321.1 flagellum-specific ATP synthase VFG0048 Protein 1e-85 50
fliI NP_243321.1 flagellum-specific ATP synthase VFG1254 Protein 2e-87 50
fliI NP_243321.1 flagellum-specific ATP synthase VFG2499 Protein 2e-81 50
fliI NP_243321.1 flagellum-specific ATP synthase VFG2331 Protein 6e-81 48
fliI NP_243321.1 flagellum-specific ATP synthase VFG0515 Protein 2e-83 48
fliI NP_243321.1 flagellum-specific ATP synthase VFG0730 Protein 4e-79 48
fliI NP_243321.1 flagellum-specific ATP synthase VFG0812 Protein 1e-78 48
fliI NP_243321.1 flagellum-specific ATP synthase VFG0555 Protein 1e-64 41