Gene Information

Name : lysS (BU437)
Accession : NP_240249.1
Strain : Buchnera aphidicola APS
Genome accession: NC_002528
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 476393 - 477913 bp
Length : 1521 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCTGAAATAGAAAAAAGAAATCATGGTGATTGTAATATTTTATACAAAGAAATAAAAAAAAGAGAACAAAAACTTGT
TAATATGAAAAAAGAAGGATTTTCTTTCCCGAATAGTTTTAAAAAAAATACTACTTCAAGAAAAATTTATCAAAAATACC
AAACAAAAAATAGAAATGAACTCATATCATTAAATATTGAAGTTTCCATTGCTGGTCGTATGGTGCAAAGACGTATTATG
GGAAAAGCTTCTTTTTTTACCTTGCAAGATATAGAAGGAAAAATACAAATTTATATAAATGAAAAAAAAATATCATCTGA
TTTTTATCGCTTTCACTTTAAAAAGTGGGATATCGGAGATATTTTGGGTGTGATAGGAACATTATTTAAAACTAAAACAG
GTGAATTATCTATTTATTGTAAAAATGTAACAATACTTAATAAATCATTAAAACCTTTACCAGACAAGTTTCATGGTTTA
TCTAATCAAGAAATACGTTATCGGAAAAGATATCTAGATTTAATTAGTAATAATAAATTATATAGTATTTTTAAAAATCG
TTCTAATATTATCACAGCGATTCGTAATTTCATGATAGAAAATGATTTTTTAGAAGTCGAAACTCCAATGCTACAAAATA
TTCCTGGAGGAGCTAATGCTCGTCCTTTTATTACTTATCATAATGAAATTGATTCTCAAATGTATTTAAGAATAGCTCCT
GAATTATATTTAAAAAAACTAATTATTGGGGGCTTTGAACGTATTTTTGAACTGAATAGAAACTTTCGTAACGAAGGAGT
ATCAGCTCGTCATAATCCAGAATTTACTATGATGGAAGCATACATTGCATATTCTAACTATGAAGATATGATGGAATTAA
CAGAAAATCTTTTTAAAAGTATTACAAAGTTTCTTTTTCAAGATAATAAAATTATATTCAACAACAATAATTTTGATTTC
AGTCAACCATTTCGTAGATTAACTATGACAGATTCTATTCTTGAATTTAATTCAACTATTACCTCATCCGATTTAAAAGA
TTTTTATAAAATCAGAGAATTTGCAAAATCTATAGGAATAAAAGTAGAAAAAAAATGGGGTTGCGGCCAGATAGAAACTG
AAATATTTGAAAAGACAGTAGAAAAAAAATTAATTCAACCAACTTTTATTACACAATACCCAGTAGAAGTTTCTCCATTG
GCAAGACGTAATGACATAAATTTAAATATAACTGATAGATTTGAACTTTTTATTGGTGGATATGAAATAGGAAATGGATT
TTCAGAACTAAATGATGTAGAAGATCAAAAAACACGATTTTTAAATCAAATTCAAAAATCAAATAAGGAAGATAATAAAA
ATCTATTTTACGATAAAGAGTATATAGAAGCGTTAAAATATGGTTTACCTCCAACATCTGGATTAGGAATAGGAATAGAT
CGTTTAATTATGATATTAACTAATCAAATCAGCATTCGTGATGTAATTTTATTTCCAACACTTCGTCCATTTAAAAAATA
G

Protein sequence :
MSEIEKRNHGDCNILYKEIKKREQKLVNMKKEGFSFPNSFKKNTTSRKIYQKYQTKNRNELISLNIEVSIAGRMVQRRIM
GKASFFTLQDIEGKIQIYINEKKISSDFYRFHFKKWDIGDILGVIGTLFKTKTGELSIYCKNVTILNKSLKPLPDKFHGL
SNQEIRYRKRYLDLISNNKLYSIFKNRSNIITAIRNFMIENDFLEVETPMLQNIPGGANARPFITYHNEIDSQMYLRIAP
ELYLKKLIIGGFERIFELNRNFRNEGVSARHNPEFTMMEAYIAYSNYEDMMELTENLFKSITKFLFQDNKIIFNNNNFDF
SQPFRRLTMTDSILEFNSTITSSDLKDFYKIREFAKSIGIKVEKKWGCGQIETEIFEKTVEKKLIQPTFITQYPVEVSPL
ARRNDINLNITDRFELFIGGYEIGNGFSELNDVEDQKTRFLNQIQKSNKEDNKNLFYDKEYIEALKYGLPPTSGLGIGID
RLIMILTNQISIRDVILFPTLRPFKK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-128 53

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS NP_240249.1 lysyl-tRNA synthetase VFG1668 Protein 1e-128 53