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Name : lysS (BSU00820) Accession : NP_387963.1 Strain : Bacillus subtilis 168 Genome accession: NC_000964 Putative virulence/resistance : Virulence Product : lysine--tRNA ligase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 88727 - 90226 bp Length : 1500 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGAGTCAAGAAGAGCATAACCATGAAGAATTGAATGATCAGCTGCAAGTCAGACGCGATAAAATGAATCAGCTGAGAGA TAACGGCATCGATCCATTCGGCGCACGTTTTGAAAGAACTCATCAGTCTCAAGAAGTTATTTCGGCATATCAAGATCTAA CCAAAGAAGAGTTAGAAGAAAAAGCGATTGAAGTTACAATCGCAGGCCGCATGATGACAAAACGCGGCAAAGGAAAAGCC GGCTTTGCCCATCTTCAGGATTTAGAAGGCCAAATCCAAATCTACGTAAGAAAAGACAGTGTCGGTGACGACCAATATGA AATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTG TTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAG CAGCGCTACCGTCAGCGCTATCTGGATCTTATCGTAAACCCAGACAGCAAACATACGTTCATTACACGAAGCAAGATCAT TCAAGCTATGAGAAGGTACCTTGATGATCATGGATACTTAGAAGTAGAAACACCTACAATGCACAGCATTCCTGGGGGAG CTTCTGCACGTCCGTTTATCACTCACCACAACGCGTTAGACATTCCACTCTATATGCGTATTGCTATCGAACTGCACCTA AAACGTCTAATTGTCGGCGGTTTAGAAAAAGTATATGAAATCGGCCGTGTTTTCCGTAACGAAGGTGTCTCTACACGCCA TAACCCTGAATTTACAATGATTGAGTTATATGAAGCATATGCGGACTATAAAGATATCATGAGCTTAACTGAAAACCTTG TCGCTCATATCGCCCAAGAAGTGCTTGGCACAACTACGATTCAATACGGGGAAGAGCAAATCGACCTTAAACCGGAGTGG AAAAGAATCCATATGGTTGACGCAGTCAAAGAAGCGACCGGCGTTGATTTCTGGGAAGAGGTTACTGTTGAGCAGGCGCG TGAATATGCAAAAGAACATGAAGTAGAAATTAAAGACTCTATGACAGTAGGCCATATCATCAACGAATTCTTCGAACAAA AAATTGAAGAAACGCTTATTCAGCCAACGTTTATTTACGGGCATCCTGTAGAAATTTCTCCTCTTGCTAAGAAAAACCCT GAGGACCCGCGTTTTACAGACCGTTTTGAGCTGTTTATCGTTGGCCGTGAACATGCCAACGCGTTTACAGAGCTGAATGA TCCTATTGATCAAAGAGAACGCTTTGAAGCGCAATTAAAAGAGCGTGAAGCCGGTAATGATGAAGCTCATTTAATGGATG AAGACTTTGTTGAAGCTCTGGAATACGGTATGCCGCCAACAGGAGGTTTAGGCATCGGTATCGACCGTCTGGTTATGCTG CTGACGAATGCCCCTTCTATTCGCGATGTGCTGTTATTCCCGCAAATGAGACAACGCTAA Protein sequence : MSQEEHNHEELNDQLQVRRDKMNQLRDNGIDPFGARFERTHQSQEVISAYQDLTKEELEEKAIEVTIAGRMMTKRGKGKA GFAHLQDLEGQIQIYVRKDSVGDDQYEIFKSSDLGDLIGVTGKVFKTNVGELSVKATSFELLTKALRPLPDKYHGLKDVE QRYRQRYLDLIVNPDSKHTFITRSKIIQAMRRYLDDHGYLEVETPTMHSIPGGASARPFITHHNALDIPLYMRIAIELHL KRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMSLTENLVAHIAQEVLGTTTIQYGEEQIDLKPEW KRIHMVDAVKEATGVDFWEEVTVEQAREYAKEHEVEIKDSMTVGHIINEFFEQKIEETLIQPTFIYGHPVEISPLAKKNP EDPRFTDRFELFIVGREHANAFTELNDPIDQRERFEAQLKEREAGNDEAHLMDEDFVEALEYGMPPTGGLGIGIDRLVML LTNAPSIRDVLLFPQMRQR |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 4e-126 | 53 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | NP_387963.1 | lysine--tRNA ligase | VFG1668 | Protein | 2e-126 | 53 |
