Gene Information

Name : dhbE (BSU31980)
Accession : NP_391078.1
Strain : Bacillus subtilis 168
Genome accession: NC_000964
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 3288641 - 3290260 bp
Length : 1620 bp
Strand : -
Note : Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; PubMedId: 12354229, 16672620, 9214294; Product type e : enzyme

DNA sequence : 
ATGCTGAAAGGATTTACGCCTTGGCCTGATGAGTTAGCTGAGACCTATCGAAAGAACGGCTGCTGGGCCGGTGAGACGTT
TGGAGATTTGTTAAGGGACCGGGCAGCCAAATATGGTGATCGCATTGCCATTACATGCGGAAACACCCATTGGAGCTATC
GAGAGCTTGACACAAGGGCTGATCGCCTGGCTGCCGGATTTCAAAAGCTCGGCATTCAACAGATGGATCGTGTTGTGGTG
CAGTTGCCAAATATCAAAGAGTTTTTTGAAGTCATTTTCGCGCTTTTCCGTCTGGGGGCGCTTCCAGTCTTCGCACTGCC
TTCTCATCGAAGCAGTGAAATTACATATTTTTGCGAGTTTGCTGAAGCGGCTGCATATATCATTCCCGACGCTTATTCCG
GTTTTGACTACCGTTCGCTTGCCAGACAGGTTCAAAGCAAGCTGCCGACTTTGAAAAACATCATTGTCGCAGGTGAGGCA
GAGGAATTTTTACCGCTGGAGGATTTGCATGCAGAACCTGTAAAACTGCCTGAAGTCAAATCATCGGATGTGGCGTTCCT
CCAGCTTTCTGGCGGGAGCACCGGGCTATCGAAACTGATTCCGAGGACCCACGATGACTACATATACAGCCTGAAGCGGA
GTGTGGAGGTCTGCTGGCTCGACCACAGCACGGTGTACTTGGCCGCGCTGCCGATGGCACACAATTATCCGCTGAGCTCA
CCCGGCGTTCTCGGCGTATTATACGCTGGAGGAAGAGTGGTATTATCTCCTTCTCCAAGTCCAGATGACGCATTTCCTTT
AATTGAACGGGAAAAGGTCACGATAACCGCTCTTGTTCCGCCTCTTGCGATGGTATGGATGGATGCGGCATCCTCACGCC
GTGATGATTTATCCAGCCTTCAAGTGCTGCAGGTCGGCGGTGCCAAGTTTAGTGCTGAAGCCGCGCGCAGGGTAAAAGCT
GTTTTCGGCTGCACGCTGCAGCAGGTGTTCGGAATGGCAGAGGGTCTCGTCAATTATACGAGATTGGATGATCCTGAGGA
GATCATTGTCAACACCCAAGGAAAACCGATGTCTCCATATGATGAAATGCGTGTTTGGGATGATCATGATCGCGACGTAA
AACCTGGTGAAACAGGCCATCTGCTGACGCGGGGGCCGTATACAATTCGAGGTTACTATAAGGCAGAAGAGCATAACGCC
GCTTCATTTACTGAGGACGGTTTTTACCGTACGGGTGATATCGTCAGGCTGACACGAGACGGCTATATTGTCGTTGAAGG
CCGGGCGAAGGATCAAATTAACCGTGGAGGAGAAAAGGTTGCGGCTGAAGAGGTAGAAAATCATCTGCTGGCGCATCCGG
CTGTCCATGATGCGGCAATGGTCTCCATGCCTGATCAATTTCTTGGCGAAAGATCTTGTGTGTTCATTATTCCCCGGGAT
GAAGCCCCAAAAGCCGCAGAGCTTAAAGCATTTTTGAGAGAGCGCGGACTGGCGGCATATAAAATCCCTGATCGAGTTGA
ATTTGTCGAATCCTTCCCGCAGACAGGAGTAGGAAAAGTCAGCAAAAAAGCGCTCCGTGAAGCCATTTCCGAGAAGCTTC
TTGCAGGATTTAAAAAATAA

Protein sequence : 
MLKGFTPWPDELAETYRKNGCWAGETFGDLLRDRAAKYGDRIAITCGNTHWSYRELDTRADRLAAGFQKLGIQQMDRVVV
QLPNIKEFFEVIFALFRLGALPVFALPSHRSSEITYFCEFAEAAAYIIPDAYSGFDYRSLARQVQSKLPTLKNIIVAGEA
EEFLPLEDLHAEPVKLPEVKSSDVAFLQLSGGSTGLSKLIPRTHDDYIYSLKRSVEVCWLDHSTVYLAALPMAHNYPLSS
PGVLGVLYAGGRVVLSPSPSPDDAFPLIEREKVTITALVPPLAMVWMDAASSRRDDLSSLQVLQVGGAKFSAEAARRVKA
VFGCTLQQVFGMAEGLVNYTRLDDPEEIIVNTQGKPMSPYDEMRVWDDHDRDVKPGETGHLLTRGPYTIRGYYKAEEHNA
ASFTEDGFYRTGDIVRLTRDGYIVVEGRAKDQINRGGEKVAAEEVENHLLAHPAVHDAAMVSMPDQFLGERSCVFIIPRD
EAPKAAELKAFLRERGLAAYKIPDRVEFVESFPQTGVGKVSKKALREAISEKLLAGFKK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 2e-116 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-116 50
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 1e-116 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-116 50
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-116 50
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-116 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-116 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-116 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-116 49

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
dhbE NP_391078.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 6e-135 55
dhbE NP_391078.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 3e-110 48
dhbE NP_391078.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 1e-111 45