Gene Information

Name : fliI (BSU16240)
Accession : NP_389506.2
Strain : Bacillus subtilis 168
Genome accession: NC_000964
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 1695877 - 1697193 bp
Length : 1317 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence : 
ATGAAGACACAGAGTCTGATAGATTGTATAGAAATGACAGACTCGTATAAACGGTACGGAAAAGTCAAGCGGGTAATCGG
CTTGATGATTGAATCAAAAGGGCCAGCAAGCTCAATTGGTGACCTGTGCCTGATTTATGCAAAAGGACAGTCTGGGAAAG
TCATTAAAGCTGAGGTAGTCGGCTTCCAGGAAGAAAATATTTTGCTTATGCCTTATTTAGAGGCTGCGAGCATTGCACCT
GGCAGCATTGTAGAAGCTACTGGGGAATCACTTCGGGTTAAAGTTGGGACCGGACTGATCGGACAAGTCATCGATGCTTT
TGGAGAACCGCTTGACGGAAAGCTTCTGCCGAAAGGGCTTTCGCCTGTATCAACGGAGCAATCACCGCCTAACCCGATGA
AACGGCCGCCTATCCGTGAAAAGATGGGTGTTGGAGTCAGATCAATTGACAGCTTGCTGACAGTCGGTAAAGGCCAGCGG
ATTGGAATTTTTGCAGGAAGCGGTGTCGGAAAAAGCACCTTAATGGGGATGATCGCCAAGCAGACAGAGGCTGACTTAAA
CGTCATTGCACTTGTCGGAGAACGCGGACGAGAGGTTCGGGAGTTTATTGAAAAAGATCTGGGGAAAGAGGGGCTGAAAC
GATCGATTGTAGTCGTTGCCACCTCAGACCAGCCAGCATTAATGAGATTGAAAGCGGCATATACAGCGACTGCAATTGCC
GAGTATTTCCGAGATAAAGGCCAAAATGTCATGTTTATGATGGATTCTGTTACAAGGGTGGCAATGGCGCAGCGAGAGAT
TGGACTGGCTGCAGGAGAGCCTCCGACGACAAAAGGCTATACACCTTCAGTGTTCGCTATTTTACCTCGTTTACTAGAAC
GAACAGGTGCAAACGAGCATGGAACCATTACGGCATTTTATACAGTGTTGGTAGATGGGGATGACATGAATGAGCCGATT
GCCGATACCGTACGCGGAATTTTAGACGGGCATATTGTGTTGGACAGGGCGCTTGCCAATAAAGGCCAGTTTCCTGCAGT
TAACGTACTAAAAAGCATCAGCAGGGTGATGTCAAACATTTCAACAAAACAGCATCTGGATGCGGCAAATAAATTCCGTG
AACTTTTATCGACATATCAAAATTCGGAAGATCTCATTAATATCGGAGCTTACAAAAGAGGATCATCCAGAGAAATTGAT
GAAGCGATACAATTTTATCCGCAGCTTATTCAATTTTTAAAGCAGGGAACAGACGAACCGGCTTTATTGGAAGAAAGCAT
TGCTGCATTAACTAGTTTGACAGGAAATGAGGAATAA

Protein sequence : 
MKTQSLIDCIEMTDSYKRYGKVKRVIGLMIESKGPASSIGDLCLIYAKGQSGKVIKAEVVGFQEENILLMPYLEAASIAP
GSIVEATGESLRVKVGTGLIGQVIDAFGEPLDGKLLPKGLSPVSTEQSPPNPMKRPPIREKMGVGVRSIDSLLTVGKGQR
IGIFAGSGVGKSTLMGMIAKQTEADLNVIALVGERGREVREFIEKDLGKEGLKRSIVVVATSDQPALMRLKAAYTATAIA
EYFRDKGQNVMFMMDSVTRVAMAQREIGLAAGEPPTTKGYTPSVFAILPRLLERTGANEHGTITAFYTVLVDGDDMNEPI
ADTVRGILDGHIVLDRALANKGQFPAVNVLKSISRVMSNISTKQHLDAANKFRELLSTYQNSEDLINIGAYKRGSSREID
EAIQFYPQLIQFLKQGTDEPALLEESIAALTSLTGNEE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-99 52
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 1e-99 52
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-99 51
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-100 51
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 6e-100 51
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 6e-100 51
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 3e-100 51
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-95 51
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-95 51
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-98 51
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 3e-93 49
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 2e-92 49
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-92 49
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-93 49
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-93 49
escN AAC38383.1 EscN Virulence LEE Protein 3e-86 48
escN YP_003236088.1 translocator EscN Virulence LEE Protein 7e-86 47
escN AAC31513.1 L0034 Virulence LEE Protein 1e-85 47
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 1e-85 47
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-85 47
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-85 47
escN CAC81862.1 EscN protein Virulence LEE II Protein 9e-85 47
escN CAI43874.1 EscN protein Virulence LEE Protein 6e-85 47
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-84 47
escN YP_003223475.1 translocator EscN Virulence LEE Protein 8e-85 47
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-92 46
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 4e-80 46
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 4e-80 46
unnamed AAL06369.1 EscN Virulence LEE Protein 5e-84 46
escN AAK26715.1 EscN Virulence LEE Protein 3e-84 46
escN AAL57542.1 EscN Virulence LEE Protein 3e-84 46
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-79 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 1e-80 45
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-81 45
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 8e-82 45
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 2e-77 44
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-79 43
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-79 43
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 2e-77 43
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 4e-78 42
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 6e-83 42
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 9e-77 41
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 6e-77 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI NP_389506.2 flagellum-specific ATP synthase VFG2144 Protein 6e-105 53
fliI NP_389506.2 flagellum-specific ATP synthase VFG0048 Protein 2e-98 51
fliI NP_389506.2 flagellum-specific ATP synthase VFG0192 Protein 3e-100 51
fliI NP_389506.2 flagellum-specific ATP synthase VFG1254 Protein 4e-100 51
fliI NP_389506.2 flagellum-specific ATP synthase VFG0390 Protein 2e-100 50
fliI NP_389506.2 flagellum-specific ATP synthase VFG0515 Protein 2e-93 49
fliI NP_389506.2 flagellum-specific ATP synthase VFG2499 Protein 3e-94 49
fliI NP_389506.2 flagellum-specific ATP synthase VFG0730 Protein 2e-86 48
fliI NP_389506.2 flagellum-specific ATP synthase VFG2331 Protein 2e-91 47
fliI NP_389506.2 flagellum-specific ATP synthase VFG0812 Protein 5e-86 47
fliI NP_389506.2 flagellum-specific ATP synthase VFG1008 Protein 1e-70 43
fliI NP_389506.2 flagellum-specific ATP synthase VFG2459 Protein 4e-78 41
fliI NP_389506.2 flagellum-specific ATP synthase VFG1778 Protein 3e-72 41