Gene Information

Name : lysS (slr1550)
Accession : NP_440803.1
Strain : Synechocystis sp. PCC 6803
Genome accession: NC_000911
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 995275 - 996807 bp
Length : 1533 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGCCGATTCCCATTCCCACTCCAGCCTTGAAGAAATCCGTGCCACCCGGCTTGAAAAAGCGGAACAATTGCGGCAACT
CGGCCTCAATCCCTATGCCTACACCTGGGAAATTACCCACCAAGCTCAGGATTTACAGGAAACCTATCGGGATCTAAGCA
ACGGCGAAGAAGTGGACCTCAAAGTGGCGATCGCCGGCCGAATCTTAGCACGGCGGGTGATGGGAAAATTAGCCTTTTTT
ACTCTCCAGGACGAAAGCGGCACCATCCAACTTTATTTGGAAAAGCAACGGCTGACGGAACATATGCCGGAGTTGGAAAA
TGCTTTTAACTTGTTGAAAAAAATCACCGATGTGGGGGATATTTTGGGGGTAACCGGCACCCTCAAACGCACGGAAAAAG
GCGAATTATCGGTTTATGTGCAAACCTACGCTGTATTAACTAAGTCTTTACTGCCCCTACCGGATAAATGGCATGGTTTG
ACTGACACAGAAAAACGCTACCGCCAACGTTACGTGGATTTAATCGTTAATCCCACCGTAAGGCAAACTTTCCGCCGCCG
GGCCCAGATTACCGCCGCCATCCGCCGTTACTTGGATAAACAGGGCTTTATCGAAATTGAAACACCAGTTTTACAGGGGG
AATCCGGGGGCGCAGAAGCTCGGCCTTTTATCACCCATCACAACACCCTGGGCATGCCCCTGTATCTCCGCATTGCTACG
GAATTACATCTGAAAAGATTGGTGGTGGGAGGCTTTGAAAAGGTTTTTGAGTTGGGGCGAATTTTTCGCAACGAAGGGGT
TTCCACCAGGCATAATCCTGAATTTACCTCCATTGAGGTCTATCAAGCCTACGTGGACTATAACGAGATGATGGCCTTAA
CCGAAGCCCTCGTCACCACCGCCGCCCAAGCAGTATTAGGCACGCTAAAAATTACCTACCAAGGGGAAGAAATCGACCTC
ACCCCTCCCTGGAAACGCATCACCATGCACGAAGCGGTGCAGTTAGAAACAGGGATAGATTTTAGCCAATTTACAGATCT
GGAAACGGCCAAACAAGCGGCCACAAAAGCGGGCATTGGTGTGCCGGAAGATTGTCCTTCCCTTGGGCATTTGTTAAACC
ATGCTTTTGAGCAAAAAGTGGAAGGGACGTTGATGCAACCCACTTTTATCATCGATTTCCCGGTGGAAATTTCTCCCCTC
GCTAAGCCCCATCGCAGTAAACCGGGGCTGGTGGAACGGTTTGAGCTATTTGTTTACGGTCGGGAATTGGCCAATAGTTT
TTCCGAGCTGACAGACCCCATTGATCAACGGAGTCGGTTGGAAGCCCAGGCGGCTAAAAAAGCGGCAGGGGATTTGGAAG
CCCACAGCGTTGATGAAGATTTCCTCACAGCGTTGGAATACGGCATGCCCCCTACTGGTGGCTTGGGCATAGGCATCGAT
CGCCTGGTGATGTTGCTGACCGATTCCCCCAGTATTCGAGATGTAATTGCTTTTCCGTTGCTGAAAAATCAGGAGGCTGG
CAGCGATGGTTGA

Protein sequence :
MADSHSHSSLEEIRATRLEKAEQLRQLGLNPYAYTWEITHQAQDLQETYRDLSNGEEVDLKVAIAGRILARRVMGKLAFF
TLQDESGTIQLYLEKQRLTEHMPELENAFNLLKKITDVGDILGVTGTLKRTEKGELSVYVQTYAVLTKSLLPLPDKWHGL
TDTEKRYRQRYVDLIVNPTVRQTFRRRAQITAAIRRYLDKQGFIEIETPVLQGESGGAEARPFITHHNTLGMPLYLRIAT
ELHLKRLVVGGFEKVFELGRIFRNEGVSTRHNPEFTSIEVYQAYVDYNEMMALTEALVTTAAQAVLGTLKITYQGEEIDL
TPPWKRITMHEAVQLETGIDFSQFTDLETAKQAATKAGIGVPEDCPSLGHLLNHAFEQKVEGTLMQPTFIIDFPVEISPL
AKPHRSKPGLVERFELFVYGRELANSFSELTDPIDQRSRLEAQAAKKAAGDLEAHSVDEDFLTALEYGMPPTGGLGIGID
RLVMLLTDSPSIRDVIAFPLLKNQEAGSDG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-101 49
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 2e-79 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS NP_440803.1 lysyl-tRNA synthetase VFG1668 Protein 8e-102 49